VCU Bioinformatics and Bioengineering Summer Institute
Virginia Commonwealth University
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Research Simulations - Sample scenario
Identification of putative protein toxin

Take a look for yourself.

Now you're really flummoxed! How is it that searching for similar sequences using your mystery DNA sequence yields no significant similarity to lef but searching for protein sequences using your translated sequence gives you perfect identity! Something must be real funny with this sequence, so you put aside fancy computer programs and just place your sequence next to the corresponding sequence from lef, aligning them by hand.

At this point who knows what to expect? What you get is the alignment shown here.

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