The protein that can't be there is.

Maybe you've encountered a novel toxin sufficiently related to lef to permit PCR amplification, but not related enough to match known lef sequences in GenBank. This doesn't make a whole lot of sense, but you don't have any better idea at the moment. With this in mind, you translate the DG47 sequence, submit the translated sequence to Blast to search for similar proteins, getting the results displayed by clicking here.

This is what you had expected a moment ago, perfect identity between your mystery protein and Bacillus proteins in the database. But that's not what you expect now. Blast just got through telling you that there wasn't any similarity between the gene and anything it knew about.

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