AlignACE 4.0 05/13/04 ./AlignACE -i cparvum_hsp.fa -numcols 10 -gcback 0.27 Parameter values: expect = 10 gcback = 0.27 minpass = 200 seed = 1181608952 numcols = 10 undersample = 1 oversample = 1 Input sequences: #0 cgd2_20 heat shock 70 (HSP70) protein #1 cgd2_1800 heat shock 40 kDa protein, putative #2 cgd2_3230 heat shock protein DnaJ Pfj2, putative #3 cgd3_3440 heat shock protein HSP70, mitochondrial #4 cgd2_3330 APG-1 like HSP70 domain containing protein, signal peptide plus likely ER retention motif #5 cgd3_3770 Hsp90 #6 cgd4_3270 heat shock 105kD; heat shock 105kD alpha; heat shock 105kD beta; heat shock 105kDa protein 1 #7 cgd6_1090 DnaJ(hsp40)'DnaJ(hsp40)' #8 cgd6_2650 heat shock protein, putative #9 cgd6_4970 Hsp60; GroEL-like chaperone (ATpase), predicted mitochondrial #10 cgd7_360 heat shock protein, Hsp70 #11 cgd7_3670 heat shock protein 90 (Hsp90), signal peptide plus ER retention motif Motif 1 GATAAGTCAAGGGGGG 5 982 1 GGATAGAAGTGGGGGA 6 844 1 GAGGGTTAATGGGGGG 5 600 1 GGTATGGCAAGGGGGA 8 33 1 GTTGAGCGATGAGGGG 7 213 0 GTGTAGTGATGGGGGA 2 416 0 GAATTTCCGCGAGGGG 4 434 1 GAAAATTTAAGAGGGG 10 1173 0 ATGCAGAAGGGAGGGG 6 998 1 GAACAAACAAGGGGGA 4 317 0 GAGCGGTTGAGGGGCA 10 805 0 GAGACGATGTGGGGAA 11 102 1 GTATCGGTGAGACGGG 5 795 1 GTTTGGTTTTGGCGGG 10 151 0 GAACAGTATCGCGGGG 11 147 0 AGCTATTTATGAGGGG 0 1645 1 AGGAATAAGAGGGGGA 0 1114 1 GACGCTACATGAGGGA 0 1230 1 ATAAATTTAAGAGGGG 10 891 1 ATATAACTAAGAGGGG 5 756 1 AAAATATGGAGGGGAG 4 459 1 GATAAAATAAGAGGCG 6 543 0 CTTAAAATACGGGGGG 4 333 1 AAATTTTGATGGGGAG 1 150 0 GGAGAAAGTAGAGGAG 4 541 1 AGTAGATATTGGGGGA 2 122 1 GTTAAGTTTTGGTGGG 2 14 0 GTATCTATTTGAGGAG 10 385 0 GAATAAAATAGGGGCA 5 1016 1 AACTGGGGAGGGCGAG 2 303 0 ATCGCGAATTGAGGAG 4 154 0 ATAAGAAAACGAGGAG 0 1043 1 ** * * ****** MAP Score: 38.6242 Motif 2 GGGGGGTTACGACGTTGT 5 610 1 GAGGGGAAAAGTCAATGT 0 1655 1 GGGGGGAAACTCAAGTTT 4 343 1 GGCGGGCTGGCACACATT 10 139 0 GGGGGGAAGTTTAAATTA 5 993 1 GAGGGGAATTCAAGTAAT 5 766 1 GGAGGGCGAGGGAAAAAA 2 295 0 GAAGGGTTTAGGCGCCAA 0 1368 1 GGCGGGAACATGCAGGAT 2 510 1 GAGGGGAGTTTCAGAATT 2 703 1 GGGGGGCCACTTTAAAAT 4 446 1 GAGGGGTTAAAACGCTTA 10 1161 0 GGCGGGAAAAGTTCTTGA 9 15 1 GCGGGGCGCGCATGAATA 11 135 0 GACGGGTACGCAAGTATT 5 805 1 GAAGGGTCAAGAAATAAT 6 716 1 GAGTGGTTTTGACACAGT 2 946 1 GAAGGGAGGGGTAAACCA 6 1003 1 GGAGGGGAGCCAAAACCT 4 466 1 GAGTGGGATAGCAGAAAA 5 630 1 GGGGAGAAAAGTCAATTT 1 138 0 GCAGGGTTGAGCTGAGAT 10 420 0 GGAGGGAGTGGATCTTAA 2 401 0 GGCGGGAACATATATTTA 2 85 1 GGGGGATATTGTAACAAA 6 854 1 GAGGGGTTGGATAACAAA 10 901 1 GAGGGGTTGTAGAAAAGA 7 201 0 TAGGGGGTTTGGCGGTAA 0 1446 1 GGGGGAATTTGTACTAAT 4 305 0 GCGTGGTTTTTGCAGTAT 6 528 0 GCCTGGTAACGCAATAAA 4 742 1 GAGTGGAATTTAAAGAGA 10 44 0 ****** * ** * MAP Score: 34.0338 Motif 3 GGGGGTAATTGTTGAGA 5 824 0 GGGGGAAAAATTTAGCG 8 43 1 GGGGGAAAATTTAAGAG 10 1176 0 GGGGGAAGAAATGAAAG 0 1124 1 GGGGGCCACTTTAAAAT 4 447 1 GGGGGGGAAGTTTAAAT 5 992 1 GGGGTTTGGCGGTAATT 0 1449 1 GGGGGAACCCTCTAAAA 6 695 1 GCGGGAACATGCAGGAT 2 511 1 AGGGGTTGTAGAAAAGA 7 201 0 GGGGGGAAACTCAAGTT 4 343 1 GAGGGGAAAAGTCAATG 0 1655 1 GGGGGATATTGTAACAA 6 854 1 AGGGGCAGGTGAGAGAA 5 1025 1 GCGGGGCGCGCATGAAT 11 136 0 GGGGGAGGGAGTGGATC 2 405 0 GCGGTTAGCTGCTAAAG 0 1001 1 TGGGGAGGGCGAGGGAA 2 299 0 GAGGGGAGTTTCAGAAT 2 703 1 GAGGGTTAATGGGGGGT 5 600 1 GGGGAGAAAAGTCAATT 1 139 0 AGGGGCAAAAGTTAAAA 10 795 0 GAGGGAAGTTTGAAATA 0 1240 1 GGGAGAAAATGAGGAAT 0 1103 1 GGGGAACTAAGGAGACG 11 112 1 TCGGGAAGATGGAAATA 2 361 1 GCGGTAAAATGGGAACA 6 1084 1 ***** * * ** * MAP Score: 19.6839 Motif 4 ACAAAAAAAAAA 0 127 0 ATTAAAAAAAAA 0 190 1 ACAAGAAAAAAA 0 323 0 AAAAGAAGAAAA 0 461 0 ATAAAAAAAAAA 0 717 0 ATTAAAAAAAAA 0 752 0 AATAGAAGAAAA 0 872 0 AATAAAAAAAAA 0 1216 1 AAAAGAAAAAAA 1 104 1 AATAGAAAAACA 2 262 0 ATCAGAAGAACA 3 463 1 AAAAGAAAAAAA 5 65 0 AGCAAAAAAAAA 5 482 1 AGTAGAAGAACA 6 813 1 AAAAAAAGAACA 6 1141 1 ATTAAAAAAAAA 7 234 1 AAAAAAAAAAAA 7 257 1 AACAAAAAAAAA 7 381 0 ATAAGAAAAAAA 7 467 1 ATGAAAAAAAAA 9 44 0 ATCAGAAAAAAA 9 95 0 ATTAAAAGAAAA 10 301 0 AGGAGAAAAAAA 10 378 0 * ********* MAP Score: 18.8244 Motif 5 GGGGGGCCACTTTAAAATATGG 4 446 1 GGGGGGTTACGACGTTGTTAGA 5 610 1 GAGGGGTTGGATAACAAATTAA 10 901 1 GAGGGCGAGGGAAAAAAGGTGG 2 290 0 GGGGGGGAAGTTTAAATTAAAA 5 992 1 GGGGGGAAACTCAAGTTTTCAG 4 343 1 GAGGGGGAAGAAATGAAAGAAA 0 1122 1 GGGGGTTTGGCGGTAATTCTGA 0 1448 1 GGGTGGAGAGCGGTTGAGGGGC 10 806 0 GAGGGAGTGGATCTTAATTTGA 2 396 0 TAGGGGCAGGTGAGAGAAGAGA 5 1024 1 GAAGGGAGGGGTAAACCATTAA 6 1003 1 GAGGGGTTGTAGAAAAGACGAA 7 197 0 CAGGGGGAACCCTCTAAAAGAG 6 693 1 GAGTGCATGCATGAAAATTTGC 5 137 1 GAATGGGAAGAGTAGTAGTAGG 0 1161 1 GAGTTGCAGGGTTGAGCTGAGA 10 421 0 CAGGGCTAACTTTAACAAGAGC 6 51 1 ****** ** ** MAP Score: 13.5139 Motif 6 TAAGAGGGGGA 0 1119 1 GATGGGGAGAA 1 148 0 GCAGGCGGGAA 2 82 1 GAGGGCGAGGG 2 301 0 GAGGGAGTGGA 2 407 0 ACAAGGGGGAA 4 316 0 TACGGGGGGAA 4 340 1 GCGAGGGGGGC 4 442 1 TATGGAGGGGA 4 463 1 TAAGAGGGGAA 5 763 1 TCAAGGGGGGG 5 988 1 GCAGGTGAGAG 5 1029 1 GAAGTGGGGGA 6 849 1 GCAGAAGGGAG 6 1000 1 GAGGGGTTGTA 7 208 0 GCAGGGAAGAA 7 510 1 GCAAGGGGGAA 8 39 1 GCAGGGTTGAG 10 427 0 GCAAGTGGGAA 10 674 0 GCGGTTGAGGG 10 808 0 GAGGGGTTGGA 10 901 1 GATGTGGGGAA 11 107 1 ** ******** MAP Score: 13.2764 Motif 7 TAAAAGAAATAAAAGG 0 236 0 GTAAATGGAAAAAAGG 0 1089 1 AAAAGGAGATAGAAGG 2 239 0 ATAAAGAGGAAAAAGG 2 723 1 GAACGGAATTAAAAGG 2 787 1 TTAAATTTTAAAAAGG 3 338 0 GTACTTTAATAGAAGG 3 579 1 GAAACTGAGAATAAGG 4 568 0 GAGCCTGTACAGAAGG 4 719 1 GGACATAAAAAAAAGG 5 236 1 GTAAATATTCAGCAGG 6 39 1 GAAATATTGAAAAAGG 6 421 0 GCAAAGAATCAAAAGG 6 496 0 GTAATTAAAGAAAAGG 9 66 1 GAGAGTCAAAAAAAGG 10 232 1 GTAAAGCTCTAGAAGG 10 268 0 GTAAGTTATTAAAAGG 10 320 0 * ** * ****** MAP Score: 12.9883 Motif 8 ATGAAGAATAATGGTC 0 1537 1 AGAAGGAATAAAGGCC 4 729 1 ACTGAGAATAAGGGAA 4 565 0 ATGGTAAATAAAGGAC 6 586 1 ATTAAAAATAATGGCC 0 736 1 ATAAAAAAAAATGGAC 0 1217 1 AAAGGAAATAAAGGTA 5 247 1 AAGAGAAAGAATGGGA 0 1153 1 AATATAAACAAAGGAA 5 41 1 AACAGGAAAAGTGGTA 2 893 1 ATAATAAACAGGGGGA 6 685 1 AAAGTAAAAAAAGAAC 6 1136 1 AAAAGAGAGAAGGGTC 6 708 1 AGTAGGAAGAAGGAAA 0 1177 1 ACAAAGAAAAAGGAAA 2 194 0 AGAGTAAAAAAGGAGA 2 246 0 AATATGGAAAGAGGTC 3 197 1 AAAAGAAAGAAAGAAA 0 267 1 AGAAAAAAAAATGAGA 10 371 0 ACAAAAAAAAAAGAAA 7 376 0 ATTAAAAAAAAAGAAA 5 69 0 ATAGTAAAGACCGGAA 5 904 1 ATGGAAAAAAGGGAGA 0 1093 1 * * *** ** ** * MAP Score: 11.8825 Motif 9 TTTGGCGCCAAT 0 354 1 TTAGGCGCCAAA 0 1375 1 TTTGGCGGTAAT 0 1453 1 TATGAGGGGAAA 0 1652 1 TTTGGGCGTAAA 2 497 0 TATGGCGCCAAA 3 477 1 TATGGGCTTAAA 4 65 1 TACGGGGGGAAA 4 340 1 TTTGGGAGGAAT 5 293 1 AATGGGGGTAAT 5 832 0 TTAGTCGCGAAT 6 463 1 TTGGGCGGTAAA 6 655 0 CTAGGCGCCAAC 6 668 1 CAAGGGGGAAAA 8 40 1 TAAGGCGCTAAA 8 53 0 TTTGGCGGGAAA 9 12 1 TGAGGGGCAAAA 10 802 0 TTGGGGGAAAAT 10 1183 0 ** ***** *** MAP Score: 10.0253 Motif 10 GGGAGAAAATGAGG 0 1103 1 GAGAAAGAATGGGA 0 1155 1 GAGTAGTAGTAGGA 0 1170 1 TAAAAATTAGGGGG 0 1439 1 GAGTAAAAAAGGAG 2 247 0 GGGAAAAAAGGTGG 2 290 0 GCATGACAAAGGAG 2 380 0 GGGGGAGGGAGTGG 2 408 0 GAGGAAAAAGGTGA 2 728 1 GAACAAACAAGGGG 4 319 0 TAAAATACGGGGGG 4 335 1 TGGTAAACAGGGAG 4 645 1 GAGGGTTAATGGGG 5 600 1 GATAAGTCAAGGGG 5 982 1 GAATAAAATAGGGG 5 1016 1 TAATAAACAGGGGG 6 686 1 GGATAGAAGTGGGG 6 844 1 GCAGAAGGGAGGGG 6 1000 1 GCGGTAAAATGGGA 6 1084 1 GAGCGATGAGGGGT 7 212 0 GAATAATAGCAGGG 7 502 1 GGTATGGCAAGGGG 8 33 1 GATGAGTTGCAGGG 10 432 0 GAGCGGTTGAGGGG 10 807 0 GAGACGATGTGGGG 11 102 1 *** ** * **** MAP Score: 7.53555 Motif 11 AAGAGGGGGA 0 1120 1 AATGGGAAGA 0 1162 1 GGGAGGGCGA 2 304 0 GGGGGAGGGA 2 412 0 TACGGGGGGA 4 340 1 AGGGGGGCCA 4 445 1 ATGGAGGGGA 4 464 1 AACAGGGAGT 4 650 1 AATGGGGGGT 5 607 1 AAGAGGGGAA 5 764 1 AAGGGGGGGA 5 990 1 AACAGGGGGA 6 691 1 AAGTGGGGGA 6 850 1 AGGGAGGGGT 6 1005 1 AAGGGGGAAA 8 41 1 AAGAGGGGTT 10 899 1 ********** MAP Score: 6.7197 Motif 12 TCGCACTTTTGGCG 0 347 1 GAAGGGTTTAGGCG 0 1368 1 TAGGGGGTTTGGCG 0 1446 1 TAATATTTTGGGCT 1 298 1 GTTAAGTTTTGGTG 2 16 0 GAGTGGTTTTGACA 2 946 1 TTAGGCTTTAGACG 3 17 0 TAGAGGTTTGGACA 3 161 0 GGGGCTTTTTGGCG 3 482 0 GGTAGGTTTTGGCT 4 473 0 GGGGGGTTACGACG 5 610 1 GCGTGGTTTTTGCA 6 532 0 GCCTAGTTTGGGCG 6 660 0 GTTTGGTTTTGGCG 10 153 0 GTCTTGTTCATGCG 10 735 0 TTAATGTTTAGGCG 10 840 0 * ***** **** MAP Score: 6.59756 Motif 13 TGCATGCAAA 0 936 0 TGCATGCAAA 3 288 1 TGCATGCAAT 0 947 1 TGGATGCAAA 5 154 0 TGCATGAAAA 6 993 0 TGCATGAAAA 5 144 1 TGCATGCATT 2 37 0 TGCATTCAAA 2 391 1 TGGTTGCAAT 6 743 1 ********** MAP Score: 5.94461 Motif 14 AGATGAGGAA 0 92 1 AAAGGGAGAA 0 1100 1 AGAGGGGGAA 0 1121 1 AGAATGGGAA 0 1160 1 AAAGTAGTAA 0 1394 0 ATGAGGGGAA 0 1653 1 AGATGGGGTA 0 1704 0 AAATGGGTAA 1 57 0 ATGGGGAGAA 1 148 0 CAGGCGGGAA 2 83 1 AAAGGAGTAT 2 377 0 CAAGGGGGAA 4 316 0 ACGGGGGGAA 4 341 1 AGAATGGGAT 5 277 1 AGAGTGGGAT 5 629 1 AAGAGGGGAA 5 764 1 AGGGGGGGAA 5 991 1 ACAGGGGGAA 6 692 1 GGAGGGGTAA 6 1007 1 AAAATGGGAA 6 1089 1 AAAGTGAGAA 7 52 0 AGAATAGGAA 8 12 0 CAAGGGGGAA 8 40 1 AAAGGAGGAA 9 77 1 AGATTGGGAA 10 208 1 AAAATGAGAA 10 370 0 AAAATAGGAA 10 454 1 AAATTGATAA 10 607 1 CAAGTGGGAA 10 674 0 AAAGTTGGAA 10 1240 1 ATGTGGGGAA 11 108 1 ********** MAP Score: 4.61677 Motif 15 AGTGAGATGGGGTA 0 1704 0 ATTTTGATGGGGAG 1 150 0 GTTTTGGTGGGCCG 2 11 0 GGGAGGGCGAGGGA 2 300 0 GGGAGGGAGTGGAT 2 406 0 GTGATGAAGCGCAG 2 816 0 ATGGAGGGGAGCCA 4 464 1 GGGTTAATGGGGGG 5 602 1 AAGTCAAGGGGGGG 5 985 1 AGTTGCATGGGCAG 6 311 0 AGTATAAAGGGCAA 6 570 0 GGATAGAAGTGGGG 6 844 1 GCGATGAGGGGTTG 7 210 0 GTTTTGGCGGGCTG 10 148 0 CGGTTGAGGGGCAA 10 804 0 GTTTAGGCGGGCAT 10 835 0 GAGACGATGTGGGG 11 102 1 AGTATCGCGGGGCG 11 145 0 *** ** **** * MAP Score: 3.29104 Motif 16 GGGAGAAAATGAGGAATAAGAGGGGG 0 1103 1 GGGAAGAGTAGTAGTAGGAAGAAGGA 0 1165 1 GGGAAATATAACGCAATAAAAAAAAA 0 1202 1 GGGAAGTTTGAAATAGTTGATAATTA 0 1242 1 GGGAAAAGTCAATGTGTCTCGTAAAG 0 1658 1 GGGGAGAAAAGTCAATTTTAAAATAA 1 130 0 GGGGGAATTAATTTGAAGTATTTGGG 2 132 1 GGTGGAATATGATAATATTAAAATAG 2 269 0 GGCAAGAGAGATGCAACTGGGGAGGG 2 307 0 GGAGGGAGTGGATCTTAATTTGAATG 2 393 0 GGGAGGTTTTACCAATTTTTGCAAAG 2 655 0 GGGGAGTTTCAGAATTAAATAAAGAG 2 705 1 GGGGAATTTGTACTAATTTCTAACTA 4 296 0 GGGGGGCCACTTTAAAATATGGAGGG 4 446 1 AGGGGGGGAAGTTTAAATTAAAATTG 5 991 1 GGCAGGTGAGAGAAGAGATAAAATCA 5 1028 1 GGGGGAACCCTCTAAAAGAGAGAAGG 6 695 1 GGGAGGGGTAAACCATTAAAATACCA 6 1006 1 GGTAAAAATAACTGAATAATGAATTG 6 1045 1 GGCGGGAAAAGTTCTTGACAGAAAAA 9 15 1 GGAGAAAAAAAAATGAGAATAAAAAG 10 363 0 GGCAAAAGTTAAAAAGTATTGTAATA 10 783 0 GGCGGGCATTATTCTTGGGTGGAGAG 10 818 0 GGGAAAATTTAAGAGGGGTTAAAACG 10 1165 0 ****** * * * * MAP Score: 2.93152 Motif 17 TAAGAGGGGGAAGA 0 1119 1 TATGAGGGGAAAAG 0 1652 1 TTTGATGGGGAGAA 1 148 0 GTGGCAGGCGGGAA 2 79 1 GGCGAGGGAAAAAA 2 295 0 GGGGAGGGAGTGGA 2 407 0 TGTGAGGAGTGGTT 2 940 1 AATACGGGGGGAAA 4 338 1 GTCAAGGGGGGGAA 5 987 1 GCAGAAGGGAGGGG 6 1000 1 GATGAGGGGTTGTA 7 208 0 GGCAAGGGGGAAAA 8 38 1 TCTAAGGAAAAGGT 10 404 1 GTTGCAGGGTTGAG 10 427 0 TTTGAGGAGTTGAT 10 443 0 GTTGAGGGGCAAAA 10 802 0 TAAGAGGGGTTGGA 10 898 1 TAAGAGGGGTTAAA 10 1168 0 GACGATGTGGGGAA 11 104 1 GGGGCGCGCATGAA 11 137 0 * ****** *** MAP Score: 1.88481 Motif 18 AAAGTTTAGTTT 1 287 0 AAAGTTGAGTTT 10 567 0 AAAGTTAAGTTT 4 517 0 AAAGTGAAATTT 10 622 1 AAAGTGTAATTT 10 355 0 AAAGTTTAATTT 2 447 1 AAAGTTGAATTT 6 339 0 CAAGTAGAGTTT 3 391 0 AAAGTAGAGGTT 3 167 0 AAAGTCAAGTAT 4 795 1 AAAGTTAATTTT 6 937 1 AAAGTCAATTTT 1 137 0 AAACTTGAGTTT 4 349 0 AAAGTCAAATAT 0 1014 1 ACAGTTAAGTTT 5 434 1 ***** ***** MAP Score: 1.27249 Motif 19 GGAATAAGAGGGGGAA 0 1115 1 AGAGAAAGAATGGGAA 0 1154 1 ACGTGGCAGGCGGGAA 2 77 1 AAGTAGATATTGGGGG 2 121 1 GGGGAGGGCGAGGGAA 2 299 0 GTGATGGGGGAGGGAG 2 411 0 TGTAAGGTACCGGTAA 2 575 1 AAGAGGAAAAAGGTGA 2 726 1 AAGTGGAAAAAGGTAG 4 183 1 AAGTTAGTAACGGTGA 5 202 1 AAGGAAATAAAGGTAA 5 248 1 ACTAAAGTGTTGGGAA 5 552 1 ATGAGGGTATCGGTGA 5 789 1 AAGTCAAGGGGGGGAA 5 985 1 AAATAGGGGCAGGTGA 5 1021 1 GGATAGAAGTGGGGGA 6 844 1 CAGAAGGGAGGGGTAA 6 1001 1 CAAAAGGTACCGGTAA 6 1034 1 GGTATGGCAAGGGGGA 8 33 1 GAGACGATGTGGGGAA 11 102 1 * * ** * ***** MAP Score: 0.99016 Motif 20 GCAATAAAAAGG 2 160 0 GCAAGCAAAAGG 4 365 0 GCAATAAAACAG 4 752 1 GAAAAAAAAATG 6 117 0 GCAACCAAATCG 6 738 0 GCAATAAAAAAG 8 20 0 GAAAAAAAAATG 9 42 0 GAAAAAAAAATG 10 374 0 **** ***** * MAP Score: 0.388378