AlignACE 4.0 05/13/04 ./AlignACE -i cparvum_hsp.fa -numcols 10 -gcback 0.27 Parameter values: expect = 10 gcback = 0.27 minpass = 200 seed = 1181608917 numcols = 10 undersample = 1 oversample = 1 Input sequences: #0 cgd2_20 heat shock 70 (HSP70) protein #1 cgd2_1800 heat shock 40 kDa protein, putative #2 cgd2_3230 heat shock protein DnaJ Pfj2, putative #3 cgd3_3440 heat shock protein HSP70, mitochondrial #4 cgd2_3330 APG-1 like HSP70 domain containing protein, signal peptide plus likely ER retention motif #5 cgd3_3770 Hsp90 #6 cgd4_3270 heat shock 105kD; heat shock 105kD alpha; heat shock 105kD beta; heat shock 105kDa protein 1 #7 cgd6_1090 DnaJ(hsp40)'DnaJ(hsp40)' #8 cgd6_2650 heat shock protein, putative #9 cgd6_4970 Hsp60; GroEL-like chaperone (ATpase), predicted mitochondrial #10 cgd7_360 heat shock protein, Hsp70 #11 cgd7_3670 heat shock protein 90 (Hsp90), signal peptide plus ER retention motif Motif 1 GATAAGTCAAGGGGGGGA 5 982 1 GAATTTCCGCGAGGGGGG 4 434 1 GTTGAGCGATGAGGGGTT 7 211 0 GTGTAGTGATGGGGGAGG 2 414 0 GGATAGAAGTGGGGGATA 6 844 1 GAGGGTTAATGGGGGGTT 5 600 1 GGTATGGCAAGGGGGAAA 8 33 1 ATGCAGAAGGGAGGGGTA 6 998 1 GAAAATTTAAGAGGGGTT 10 1171 0 GTATCGGTGAGACGGGTA 5 795 1 GATAAAATAAGAGGCGTG 6 541 0 GAGCGGTTGAGGGGCAAA 10 803 0 GAACAAACAAGGGGGAAT 4 315 0 GTTTGGTTTTGGCGGGCT 10 149 0 GACGCTACATGAGGGAAG 0 1230 1 ACAGTATCGCGGGGCGCG 11 143 0 GAGACGATGTGGGGAACT 11 102 1 ATATAACTAAGAGGGGAA 5 756 1 AGGAATAAGAGGGGGAAG 0 1114 1 AGCTATTTATGAGGGGAA 0 1645 1 TTTATATATTGAGGGGAG 2 693 1 TTAAAATATGGAGGGGAG 4 457 1 ATAAATTTAAGAGGGGTT 10 891 1 GAATAAAATAGGGGCAGG 5 1016 1 ACTAAATTGTGAGGAGTG 2 933 1 GGAGAAAGTAGAGGAGTT 4 541 1 GTATCTATTTGAGGAGAA 10 383 0 TGGGGAGGGCGAGGGAAA 2 298 0 TTAATGTTTAGGCGGGCA 10 836 0 AAATTTTGATGGGGAGAA 1 148 0 AGTAGATATTGGGGGAAT 2 122 1 CTTAAAATACGGGGGGAA 4 333 1 ATAAGAAAACGAGGAGTT 0 1043 1 * * * ****** * MAP Score: 37.2746 Motif 2 GGAGGGCGAGG 2 302 0 GCGAGGGGGGC 4 442 1 GCGGGGCGCGC 11 142 0 AGGGGGGGAAG 5 991 1 TGGAGGGGAGC 4 465 1 AGAGGGGGAAG 0 1121 1 GGGAGGGGTAA 6 1006 1 ACAGGGGGAAC 6 692 1 AGAGGGGTTGG 10 900 1 ACGGGGGGAAA 4 341 1 GGCAGGCGGGA 2 81 1 GCAAGGGGGAA 8 39 1 GGCGGGCTGGC 10 146 0 TGATGGGGAGA 1 149 0 GGAGGGAGTGG 2 408 0 TGGGGGGTTAC 5 609 1 AGAGGGGTTAA 10 1169 0 ACAAGGGGGAA 4 316 0 GCATGGGCAGG 6 310 0 TGAGGGGCAAA 10 803 0 AGATGGGGTAA 0 1703 0 GCAGGGCTAAC 6 50 1 TGCAGGGTTGA 10 428 0 TGGTGGGCCGA 2 10 0 AGAAGGGTCAA 6 715 1 GGGTGGAGAGC 10 817 0 ******** ** MAP Score: 27.4533 Motif 3 GGGAAGAGTAG 0 1165 1 GGGAAGTTTGA 0 1242 1 GGCAGGCGGGA 2 81 1 GGCGAGGGAAA 2 298 0 GGCAAGAGAGA 2 322 0 GGGAAGATGGA 2 363 1 TGGGGGAGGGA 2 412 0 GTGAGGAGTGG 2 941 1 TGCAAGTGGAA 4 180 1 CGCGAGGGGGG 4 441 1 TGGAGGGGAGC 4 465 1 TGCAAGCGTGA 5 321 1 GTCAAGGGGGG 5 987 1 GGCAGGTGAGA 5 1028 1 GGGAGGGGTAA 6 1006 1 GGCAAGGGGGA 8 38 1 GGCGGGCTGGC 10 146 0 TGCAGGGTTGA 10 428 0 TGCAAGTGGGA 10 675 0 GGGTGGAGAGC 10 817 0 ****** **** MAP Score: 23.3664 Motif 4 CTTTTGGCGCCAA 0 352 1 AACATGGCGGTTA 0 995 1 GTTTAGGCGCCAA 0 1373 1 GGTTTGGCGGTAA 0 1451 1 TGGCAGGCGGGAA 2 80 1 GATATTGGGGGAA 2 126 1 CTGGGGAGGGCGA 2 304 0 CAAAAGGCGGGAA 2 505 1 CCTATGGCGCCAA 3 475 1 AATACGGGGGGAA 4 338 1 GCGAGGGGGGCCA 4 442 1 GTCAAGGGGGGGA 5 987 1 GATTAGTCGCGAA 6 461 1 GTTTGGGCGGTAA 6 656 0 CTGTTGGCGCCTA 6 669 0 GGCAAGGGGGAAA 8 38 1 AATAAGGCGCTAA 8 54 0 ATTTTGGCGGGAA 9 10 1 TGGCGGGCTGGCA 10 145 0 GTTGAGGGGCAAA 10 803 0 GTTTAGGCGGGCA 10 836 0 ACGATGTGGGGAA 11 105 1 CGCGGGGCGCGCA 11 141 0 * * ******** MAP Score: 19.7483 Motif 5 ACAAAAAAAAAA 0 127 0 ATTAAAAAAAAA 0 190 1 ACAAGAAAAAAA 0 323 0 AAAAGAAGAAAA 0 461 0 ATAAAAAAAAAA 0 717 0 ATTAAAAAAAAA 0 752 0 AATAGAAGAAAA 0 872 0 AATAAAAAAAAA 0 1216 1 AAAAGAAAAAAA 1 104 1 AATAGAAAAACA 2 262 0 ATCAGAAGAACA 3 463 1 AAAAGAAAAAAA 5 65 0 AGCAAAAAAAAA 5 482 1 AGTAGAAGAACA 6 813 1 AAAAAAAGAACA 6 1141 1 ATTAAAAAAAAA 7 234 1 AATAAAAAAAAA 7 254 1 ACAAAAAAAAAA 7 380 0 ATAAGAAAAAAA 7 467 1 ATGAAAAAAAAA 9 44 0 ATCAGAAAAAAA 9 95 0 ATTAAAAGAAAA 10 301 0 AGGAGAAAAAAA 10 378 0 * ********* MAP Score: 18.8244 Motif 6 GAACGGAATTAAAAGGTAA 2 787 1 GCAAAGAATCAAAAGGTAA 6 493 0 GGCGAGGGAAAAAAGGTGG 2 290 0 GGTAGGATTCAAAAGTTAA 6 926 1 GGAGAGATAGAAAAGTAGA 4 593 1 GCATAGACGTAAAAGTAAA 5 922 1 GTAAAGTGATAAAAGTAAA 0 1075 1 GTTGAGGGGCAAAAGTTAA 10 797 0 GATTGGCGCCAAAAGTGCG 0 348 0 GAATGGTAAATAAAGGACA 6 584 1 GTAAAGCTCTAGAAGGTCA 10 265 0 TAAAAGAAATAAAAGGCAA 0 233 0 ATAAAGAGGAAAAAGGTGA 2 723 1 GGTTAGCTGCTAAAGTCAA 0 1003 1 TGCAAGTGGAAAAAGGTAG 4 180 1 GTTAAGTTTTAAAATGGAA 5 437 1 TGATGGGGAGAAAAGTCAA 1 141 0 AATGAGAATAAAAAGTGTA 10 359 0 AAAAAGGAAATAAAGGTAA 5 245 1 GCAAGGGGGAAAAATTTAG 8 39 1 AGTGAGACTTTAAAGGCAG 4 121 0 * ** ****** * MAP Score: 17.565 Motif 7 GCATGCACTC 5 137 0 GCATGCAAAA 3 289 1 GCATGCAAAA 0 935 0 GCATGCAATC 0 948 1 GCTTGCATAC 5 360 1 GCATGCATTC 2 36 0 GCTTGCATAA 5 318 0 GCATGAACAA 0 984 1 GCATGAACAA 10 736 1 GCTGGCACAC 10 142 0 GCATGAAAAA 6 992 0 GCTTGTACTA 10 485 0 GCTTGTAAAA 0 591 0 GCAAGCAAAA 4 367 0 GCATGAATAA 11 134 0 GCTTGTAATA 7 150 0 GCTTGTAATA 10 117 1 ********** MAP Score: 11.2108 Motif 8 AAATGGAAAAAAGGGAGAAAATGAG 0 1091 1 AAATAAAAATTAGGGGGTTTGGCGG 0 1436 1 ATCTAGTGAGATGGGGTAACTTGTA 0 1697 0 AAATGTTGAGAAGTGGTTATTTTAA 1 114 1 GTGTGTAGTGATGGGGGAGGGAGTG 2 409 0 ATATTTATATAGGGAGGTTTTACCA 2 667 0 TTATATATTGAGGGGAGTTTCAGAA 2 694 1 ATTTTGCTATACTGGGGCTTTTTGG 3 484 0 TCCTTAAAATACGGGGGGAAACTCA 4 331 1 AATTTCCGCGAGGGGGGCCACTTTA 4 435 1 AAATATAACTAAGAGGGGAATTCAA 5 754 1 AGATAAGTCAAGGGGGGGAAGTTTA 5 981 1 ATTTGGATAGAAGTGGGGGATATTG 6 840 1 AAGTTGAGCGATGAGGGGTTGTAGA 7 206 0 AATTAAAGAAAAGGAGGAACTTTGG 9 68 1 TAATAAATTTAAGAGGGGTTGGATA 10 889 1 TAATTTTTCTTTGGGGGAAAATTTA 10 1180 0 TCTTGAGACGATGTGGGGAACTAAG 11 98 1 * * ** ***** * MAP Score: 7.76862 Motif 9 GCCAAAAGTGCGAGAA 0 344 0 GCAAAAACTGTAATTA 0 409 0 GCAATCAATGTGGACA 0 952 1 GCCAAATCGAGAGTTA 0 1381 1 GCAAAATTTGATACTA 1 342 1 GCCCAAAAGGCGGGAA 2 502 1 GCAAAAATTGGTAAAA 2 659 1 GCCATAAAGGATACGA 4 413 0 GCCCCCCTCGCGGAAA 4 437 0 GCCAAAATTAAGGTTA 4 501 1 GCCAAATAAATGGTGA 5 955 1 GCCAAAAGTGAGAATA 7 50 0 GCCATACCGGCAATAA 8 25 0 GCCAAAATAAATGTCA 9 2 0 GCCAAATCAAGAAGGA 10 337 0 GCAAACTCCGTGAGAA 10 592 1 GCCCACATAATTAATA 10 925 1 ****** * ** * MAP Score: 5.52671 Motif 10 GGCGCCAAAA 0 353 0 GGCGCCTAAA 0 1374 0 ACCGCCAAAC 0 1452 0 GGCGGGAACA 2 85 1 CGAGGGAAAA 2 297 0 GGCGGGAACA 2 510 1 GACGAGCAAA 3 11 0 GGCGCCAAAA 3 480 1 GGAGCCAAAA 4 471 1 ACCGCCCAAA 6 658 1 GGCGCCAACA 6 671 1 CAAGCCAAAA 7 59 0 GCAGGGAAGA 7 510 1 AGGGGGAAAA 8 42 1 CCCGCCAAAA 9 11 0 CCCGCCAAAA 10 151 1 AGGGGCAAAA 10 802 0 CCCGCCTAAA 10 838 1 CCCGCGATAC 11 148 1 ********** MAP Score: 5.17219 Motif 11 AGAAAACGAGGAG 0 1046 1 GGAAAAAAGGGAG 0 1095 1 GGAATAAGAGGGG 0 1115 1 GAGAAAGAATGGG 0 1155 1 GAAAATTGAAGGG 0 1361 1 AAAAATTAGGGGG 0 1440 1 AGTAATAAATGGG 1 60 0 AGTAAAAAAGGAG 2 247 0 GGGAAAAAAGGTG 2 291 0 GGGGAGGGAGTGG 2 408 0 ATATATTGAGGGG 2 696 1 GAGGAAAAAGGTG 2 728 1 AAATTGTGAGGAG 2 936 1 GCGAATTGAGGAG 4 154 0 ACAAACAAGGGGG 4 318 0 AAAATACGGGGGG 4 336 1 AAATATGGAGGGG 4 460 1 GAAAGTAGAGGAG 4 544 1 GAGAATAAGGGAA 4 565 0 GGTAAACAGGGAG 4 646 1 AGAAATTTGGGAG 5 288 1 GGTTAATGGGGGG 5 603 1 GGGTATCGGTGAG 5 793 1 AATAAATGGTGAG 5 959 1 ATAAGTCAAGGGG 5 983 1 AATAAAATAGGGG 5 1017 1 ATTGAAAAAGGAG 6 419 0 AATAAACAGGGGG 6 687 1 GGATAGAAGTGGG 6 844 1 AAAAAATAAGGCG 8 58 0 AGGAGTTGATGAG 10 440 0 ATGAATTGGTGAG 10 546 0 AATTTAAGAGGGG 10 894 1 AATTTAAGAGGGG 10 1173 0 GGGAACTAAGGAG 11 113 1 ** ** ****** MAP Score: 4.58378 Motif 12 TTTGATGGGGAG 1 150 0 TTTGGTGGGCCG 2 11 0 TGGGGAGGGCGA 2 304 0 TGGGGGAGGGAG 2 411 0 TTGCATGGGCAG 6 311 0 TAGTTTGGGCGG 6 659 0 TTTGGCGGGCTG 10 148 0 TTGGGTGGAGAG 10 818 0 TATCGCGGGGCG 11 145 0 ***** **** * MAP Score: 2.18951 Motif 13 ATAAGAAAACGAGGAG 0 1043 1 GGGAGAAAATGAGGAA 0 1103 1 GAGAAAGAATGGGAAG 0 1155 1 GGGAGGGCGAGGGAAA 2 298 0 ATGGGGGAGGGAGTGG 2 408 0 GAGGGTTAATGGGGGG 5 600 1 ATAACTAAGAGGGGAA 5 758 1 GTATCGGTGAGACGGG 5 795 1 AAGTCAAGGGGGGGAA 5 985 1 GGGCAGGTGAGAGAAG 5 1027 1 GGATAGAAGTGGGGGA 6 844 1 ATGCAGAAGGGAGGGG 6 998 1 TGAGCGATGAGGGGTT 7 211 0 GTATGGCAAGGGGGAA 8 34 1 TTAAAGAAAAGGAGGA 9 70 1 GAGCGGTTGAGGGGCA 10 805 0 TTAAAGAAGAGAGGTA 10 958 1 GAGACGATGTGGGGAA 11 102 1 * * ** * **** * MAP Score: 2.03166 Motif 14 TAAGAGGGGGA 0 1119 1 AATTAGGGGGT 0 1443 1 TATGAGGGGAA 0 1652 1 GCAGGCGGGAA 2 82 1 TATTGGGGGAA 2 128 1 AAAGGCGGGAA 2 507 1 TATTGAGGGGA 2 699 1 TACGGGGGGAA 4 340 1 TATGGAGGGGA 4 463 1 TAATGGGGGGT 5 606 1 TAAGAGGGGAA 5 763 1 TCAAGGGGGGG 5 988 1 AAACAGGGGGA 6 690 1 GAAGTGGGGGA 6 849 1 AAGCGAGCGAA 7 156 1 GAGTTAGGGAA 7 494 1 GCAAGGGGGAA 8 39 1 AAAAGGAGGAA 9 76 1 GAAGAGAGGTA 10 963 1 GATGTGGGGAA 11 107 1 ** ******** MAP Score: 1.73788 Motif 15 AAAAGGGAGAAAATGAG 0 1099 1 GAATGGGAAGAGTAGTA 0 1161 1 AATTAGGGGGTTTGGCG 0 1443 1 ATGAGGGGAAAAGTCAA 0 1653 1 AGATGGGGTAACTTGTA 0 1697 0 ATTTGGGAGCCTTTTTA 2 151 1 AAAAGGAGATAGAAGGA 2 238 0 ACTGGGGAGGGCGAGGG 2 301 0 ATGGGGGAGGGAGTGGA 2 407 0 AAAGGCGGGAACATGCA 2 507 1 ATATGGAAAGAGGTCAA 3 198 1 ACAAGGGGGAATTTGTA 4 310 0 ATACGGGGGGAAACTCA 4 339 1 CGAGGGGGGCCACTTTA 4 443 1 ATATGGAGGGGAGCCAA 4 462 1 ATTTGGGAGGAATACAA 5 292 1 AGACGGGTACGCAAGTA 5 804 1 AAATGGGGGTAATTGTT 5 828 0 AAGGGGGGGAAGTTTAA 5 990 1 AAATAGGGGCAGGTGAG 5 1021 1 ACAGGGGGAACCCTCTA 6 692 1 AAGTGGGGGATATTGTA 6 850 1 AAGGGAGGGGTAAACCA 6 1004 1 ATGTGGGGAACTAAGGA 11 108 1 * * ****** * * MAP Score: 1.4282 Motif 16 ACGAGGAGTTG 0 1051 1 AAGAGGGGGAA 0 1120 1 AAGAGTAGTAG 0 1168 1 ATTAGGGGGTT 0 1444 1 AGGAGTGGTTT 2 944 1 AGTAGGGTTTA 3 95 0 AGTAGAGGTTT 3 166 0 AATGGGGGGTT 5 607 1 AATGGGGGTAA 5 833 0 AAGGGGGGGAA 5 990 1 AGGCGTGGTTT 6 537 0 GGGAGGGGTAA 6 1006 1 ATGAGGGGTTG 7 210 0 ATTAGTGGTAA 10 877 1 AAGAGGGGTTG 10 899 1 AATAGCGGTTA 10 998 1 AAGAGGGGTTA 10 1170 0 * ********* MAP Score: 0.541493 Motif 17 GGGGTAACTTGTA 0 1697 0 GAGGGGAGTTTCA 2 703 1 GGGGGAATTTGTA 4 310 0 GGGGGTAATTGTT 5 828 0 GGGGGGAAGTTTA 5 993 1 GGGGGAAAATTTA 10 1180 0 ***** * **** MAP Score: 0.447717 Motif 18 AAGAGGGGGAA 0 1120 1 GGGCGAGGGAA 2 299 0 GAGAGATGCAA 2 317 0 GGAAGATGGAA 2 364 1 GAGGGAGTGGA 2 407 0 GAAAGAGGTCA 3 203 1 GAGGGGGGCCA 4 444 1 GAGGGGAGCCA 4 467 1 GAATAAGGGAA 4 565 0 GAGTGGGATAG 5 630 1 AAGGGGGGGAA 5 990 1 GGGCAGGTGAG 5 1027 1 GAGAAGGGTCA 6 714 1 GGGAGGGGTAA 6 1006 1 GAGTGAGTCAA 6 1116 1 GAGGGGTTGTA 7 208 0 GCAAGGGGGAA 8 39 1 GAGAGAGTCAA 10 230 1 GAAAGATTGAA 10 749 0 GAGCGGTTGAG 10 810 0 GAGGGGTTGGA 10 901 1 *** ******* MAP Score: 0.332206