AlignACE 4.0 05/13/04 ./AlignACE -i cparvum_hsp.fa -numcols 10 -gcback 0.27 Parameter values: expect = 10 gcback = 0.27 minpass = 200 seed = 1181608875 numcols = 10 undersample = 1 oversample = 1 Input sequences: #0 cgd2_20 heat shock 70 (HSP70) protein #1 cgd2_1800 heat shock 40 kDa protein, putative #2 cgd2_3230 heat shock protein DnaJ Pfj2, putative #3 cgd3_3440 heat shock protein HSP70, mitochondrial #4 cgd2_3330 APG-1 like HSP70 domain containing protein, signal peptide plus likely ER retention motif #5 cgd3_3770 Hsp90 #6 cgd4_3270 heat shock 105kD; heat shock 105kD alpha; heat shock 105kD beta; heat shock 105kDa protein 1 #7 cgd6_1090 DnaJ(hsp40)'DnaJ(hsp40)' #8 cgd6_2650 heat shock protein, putative #9 cgd6_4970 Hsp60; GroEL-like chaperone (ATpase), predicted mitochondrial #10 cgd7_360 heat shock protein, Hsp70 #11 cgd7_3670 heat shock protein 90 (Hsp90), signal peptide plus ER retention motif Motif 1 GATAAGTCAAGGGGGG 5 982 1 GGATAGAAGTGGGGGA 6 844 1 GTTGAGCGATGAGGGG 7 213 0 GAGGGTTAATGGGGGG 5 600 1 GGTATGGCAAGGGGGA 8 33 1 GAATTTCCGCGAGGGG 4 434 1 GTGTAGTGATGGGGGA 2 416 0 ATGCAGAAGGGAGGGG 6 998 1 GTATCGGTGAGACGGG 5 795 1 GTTTGGTTTTGGCGGG 10 151 0 GAAAATTTAAGAGGGG 10 1173 0 GAGACGATGTGGGGAA 11 102 1 GAACAGTATCGCGGGG 11 147 0 GAACAAACAAGGGGGA 4 317 0 GAGCGGTTGAGGGGCA 10 805 0 CTTAAAATACGGGGGG 4 333 1 AGCTATTTATGAGGGG 0 1645 1 AGGAATAAGAGGGGGA 0 1114 1 AAAATATGGAGGGGAG 4 459 1 ATATAACTAAGAGGGG 5 756 1 GACGCTACATGAGGGA 0 1230 1 ATAAATTTAAGAGGGG 10 891 1 GTTAAGTTTTGGTGGG 2 14 0 GGAGAAAGTAGAGGAG 4 541 1 GATAAAATAAGAGGCG 6 543 0 AAATTTTGATGGGGAG 1 150 0 AGTAGATATTGGGGGA 2 122 1 GTATCTATTTGAGGAG 10 385 0 AACTGGGGAGGGCGAG 2 303 0 ATCGCGAATTGAGGAG 4 154 0 CATTTATTTTGGCGGG 9 5 1 GAATAAAATAGGGGCA 5 1016 1 ** * * ****** MAP Score: 38.167 Motif 2 GCGAGGGGGGCC 4 442 1 TGGAGGGGAGCC 4 465 1 GGGAGGGGTAAA 6 1006 1 GCATGGGCAGGA 6 309 0 TCAAGGGGGGGA 5 988 1 TGGCGGGCTGGC 10 146 0 TGATGGGGAGAA 1 148 0 GCAAGGGGGAAA 8 39 1 AGAGGGGGAAGA 0 1121 1 GCGGGGCGCGCA 11 141 0 ACGGGGGGAAAC 4 341 1 AGATGGGGTAAC 0 1702 0 TGGTGGGCCGAA 2 9 0 GGAGGGCGAGGG 2 301 0 ACAGGGGGAACC 6 692 1 TGATGGGGGAGG 2 414 0 GGCAGGCGGGAA 2 81 1 TGAGGGGCAAAA 10 802 0 AGAAGTGGGGGA 6 848 1 AGCAGGGCTAAC 6 49 1 GCAAGTGGAAAA 4 181 1 GCAAGTGGGAAA 10 673 0 TCATGTGGAAAA 4 95 0 *** **** *** MAP Score: 23.9411 Motif 3 CTTTTGGCGCC 0 352 1 GTTTAGGCGCC 0 1373 1 GGTTTGGCGGT 0 1451 1 GTTTTGGTGGG 2 14 0 ATTTGGGAGCC 2 151 1 TTTTTGGCGCC 3 480 0 GTTTTGGCTCC 4 471 0 GTTTAGGAGAG 4 588 1 GTTTGGGCGGT 6 658 0 CTGTTGGCGCC 6 671 0 ATTTTGGCGGG 9 10 1 GTTTTGGCGGG 10 151 0 GTTTAGGCGGG 10 838 0 **** ****** MAP Score: 20.529 Motif 4 AAAAGAAAGAAAGA 0 267 1 AAATGAAAGAAAGA 0 1132 1 ATAAGAAAAAAATA 7 467 1 ATCAGAAAAAAACA 9 93 0 AGGAGAAAAAAAAA 10 376 0 ATAGGAAAAAAATA 0 568 0 ATTCGAAAAAAAAA 6 119 0 ACTCGAAAAAAAAA 7 112 0 AATAGAAGAAAATA 0 870 0 AACTGAAGAAAATA 10 70 0 AATTAAAAGAAATA 0 241 0 AATTAAAAGAAAAA 10 300 0 ACAAAAAAGAAAAA 1 100 1 AAAAAAAAGAAAAA 5 67 0 ATAAAAAAAAAAAA 7 255 1 ACAAAAAAAAAAGA 7 378 0 AACAAAAAAAAAAA 0 126 0 ATGGAAAAAAAATA 0 1341 0 AAAAAAAAAAAAAA 7 237 1 ATAAAAAAAAAATA 0 715 0 AAATAAAGGAAAGA 10 754 0 AGAAAAAGGAAAAA 2 192 0 AAAAAAAGGAAATA 5 243 1 AATTAAAGAAAATA 4 213 0 ATTAAAAAAAAAGG 0 750 0 AATAAAAAAAAATG 0 1216 1 ATGAAAAAAAAATG 9 42 0 ATTGAAAGGAAATG 5 671 0 AATTAAAGAAAAGG 9 68 1 * ******** * MAP Score: 18.1631 Motif 5 AATAAATGGGTAAGGT 1 54 0 AAAAAAAGGGTAATTT 5 1061 1 AAACAAGGGGGAATTT 4 313 0 AAATAAAAGGCAATTT 0 230 0 AAAGAATAGGAAAAAT 8 8 0 AAGAAAAAGGAAAAAT 2 191 0 AATCAAAAGGTAATGT 6 490 0 AATAAACAGGAAAAGT 2 889 1 AACTAAGAGGGGAATT 5 760 1 AAAGAATGGGAAGAGT 0 1158 1 AATAAAGAGGAAAAAG 2 722 1 AATTAAAAGGTAATAG 2 793 1 AAAAAAGGGAGAAAAT 0 1097 1 AAGAAAAGGAGGAACT 9 73 1 AATAAATGGAAGAATT 6 288 1 AAACAAAGGAAGACAT 5 46 1 AATAAAATGGTAAAAT 0 803 0 AAATAAATGGTGAGAT 5 958 1 AATTAAAAGAAAAAAT 10 298 0 AAATAAAGGAAAGATT 10 752 0 ** ** *** ** * MAP Score: 15.8783 Motif 6 CTTTTGGCGCCAA 0 352 1 TAAGAGGGGGAAG 0 1119 1 GTTTAGGCGCCAA 0 1373 1 GGTTTGGCGGTAA 0 1451 1 TGGCAGGCGGGAA 2 80 1 GTGATGGGGGAGG 2 414 0 CAAAAGGCGGGAA 2 505 1 CCTATGGCGCCAA 3 475 1 AAACAAGGGGGAA 4 316 0 AATACGGGGGGAA 4 338 1 GCGAGGGGGGCCA 4 442 1 GGAGGGGAGCCAA 4 466 1 TAATGGGGGGTTA 5 606 1 TAAATGGGGGTAA 5 833 0 GTCAAGGGGGGGA 5 987 1 GACAAAGCGTCAA 6 599 1 GTTTGGGCGGTAA 6 656 0 CTGTTGGCGCCTA 6 669 0 GAAGTGGGGGATA 6 849 1 GGCAAGGGGGAAA 8 38 1 AATAAGGCGCTAA 8 54 0 ATTTTGGCGGGAA 9 10 1 GTTTTGGCGGGCT 10 149 0 GATTTGGCGTAAA 10 345 1 GTTGAGGGGCAAA 10 803 0 GTTTAGGCGGGCA 10 836 0 CTAATAGCGGTTA 10 996 1 TAAGAGGGGTTAA 10 1169 0 TCTTTGGGGGAAA 10 1185 0 CGCGGGGCGCGCA 11 141 0 * ********* MAP Score: 14.2346 Motif 7 ATAAGAAAACGAGGAGTTG 0 1043 1 GAGGAATAAGAGGGGGAAG 0 1113 1 GAAAGAATGGGAAGAGTAG 0 1157 1 GAAAAAAAATGTTGAGAAG 1 108 1 TAAAAATTTTGATGGGGAG 1 150 0 GTTAAGTTTTGGTGGGCCG 2 11 0 GAGAGATGCAACTGGGGAG 2 309 0 GTGATGGGGGAGGGAGTGG 2 408 0 TTAAAATATGGAGGGGAGC 4 457 1 GGAGAAAGTAGAGGAGTTT 4 541 1 TAAAAATAATCTGGAGAAG 5 571 1 GTGAGGGTTAATGGGGGGT 5 598 1 GAAAATGAATATTGAGAAG 5 643 1 TGAGGGTATCGGTGAGACG 5 790 1 TAGATAAGTCAAGGGGGGG 5 980 1 TTGAATAAAATAGGGGCAG 5 1014 1 TTGGATAGAAGTGGGGGAT 6 842 1 GTTGAGCGATGAGGGGTTG 7 210 0 GTGAATTAATGATGTGATT 7 292 1 GAAAAAAATAAGTGAGTGG 7 471 1 GGGAATAATAGCAGGGAAG 7 500 1 GCAGGGTTGAGCTGAGATG 10 419 0 GTGGAGAGCGGTTGAGGGG 10 807 0 ATAAATTTAAGAGGGGTTG 10 891 1 GGGGAAAATTTAAGAGGGG 10 1173 0 TTGAGACGATGTGGGGAAC 11 100 1 * *** * **** * MAP Score: 11.2431 Motif 8 GCATGCACTC 5 137 0 GCATGCAAAA 0 935 0 GCATGCAAAA 3 289 1 GCATGCATTC 2 36 0 GCTTGCATAC 5 360 1 GCATGCAATC 0 948 1 GCATGAACAA 0 984 1 GCATGAACAA 10 736 1 GCTGGCACAC 10 142 0 GCTTGCATAA 5 318 0 GCATGAAAAA 6 992 0 GCATGAATAA 11 134 0 GCAAGCAAAA 4 367 0 ********** MAP Score: 10.8955 Motif 9 ATAAGAAAACGAGGAG 0 1043 1 GAGGAATAAGAGGGGG 0 1113 1 GAAGGAAACAATGTAG 0 1185 1 AAATTTTGATGGGGAG 1 150 0 AAGAGTAAAAAAGGAG 2 247 0 GAGGGAAAAAAGGTGG 2 290 0 AGATGCAACTGGGGAG 2 309 0 ATGCATGACAAAGGAG 2 380 0 GGGGGAGGGAGTGGAT 2 406 0 AAGTGATGAAGCGCAG 2 816 0 AAATTGTGAGGAGTGG 2 936 1 AAGTGGAAAAAGGTAG 4 183 1 AGGAACAAACAAGGGG 4 319 0 AAAATATGGAGGGGAG 4 459 1 GGAGAAAGTAGAGGAG 4 541 1 GAGGGTTAATGGGGGG 5 600 1 ATAAGTCAAGGGGGGG 5 983 1 TTGGATAGAAGTGGGG 6 842 1 ATGCAGAAGGGAGGGG 6 998 1 GAGCGATGAGGGGTTG 7 210 0 AAAAATAAGTGAGTGG 7 474 1 GGGAATAATAGCAGGG 7 500 1 GAGAGCGGTTGAGGGG 10 807 0 GAAAATTTAAGAGGGG 10 1173 0 TTGAGACGATGTGGGG 11 100 1 GAACAGTATCGCGGGG 11 147 0 *** * * * **** MAP Score: 9.17524 Motif 10 GTGCGAGAATTTC 0 340 0 AAGGGAGAAAATG 0 1101 1 GAGGGGGAAGAAA 0 1122 1 ATGAGGGAAGTTT 0 1238 1 GAGGGGAAAAGTC 0 1655 1 ATGGGGAGAAAAG 1 145 0 AGGCGGGAACATA 2 84 1 TTGGGGGAATTAA 2 130 1 AAGGTGGAATATG 2 284 0 AGGCGGGAACATG 2 509 1 AAGGGGGAATTTG 4 312 0 ACGGGGGGAAACT 4 341 1 ATGGGGGGTTACG 5 608 1 AAGAGGGGAATTC 5 764 1 ATGGGGGTAATTG 5 830 0 AGGGGGGGAAGTT 5 991 1 GTGGGGGATATTG 6 852 1 GTGCGGTAAAATG 6 1082 1 ATGAGGGGTTGTA 7 208 0 AAGGGGGAAAAAT 8 41 1 GCGGGAAAAGTTC 9 16 1 AAGGAGGAACTTT 9 78 1 AAGTGGGAAATTG 10 670 0 GGGGGAAAATTTA 10 1180 0 * ******* ** MAP Score: 8.54956 Motif 11 TGATGGGGAG 1 150 0 GGCAGGCGGG 2 81 1 GGAGGGCGAG 2 303 0 GGCAAGAGAG 2 323 0 GGGAAGATGG 2 363 1 GAGGGAGTGG 2 408 0 TTGAGGGGAG 2 701 1 GGAAAAGTGG 2 897 1 TGAGGAGTGG 2 942 1 GGAAAGGTGG 4 7 0 CGCGAGGGGG 4 441 1 ATATGGAGGG 4 462 1 TAATGGGGGG 5 606 1 GTGAGACGGG 5 801 1 GTCAAGGGGG 5 987 1 GGCAGGTGAG 5 1028 1 GAGAGAAGGG 6 712 1 TGCAGAAGGG 6 999 1 CGATGAGGGG 7 213 0 GGCAAGGGGG 8 38 1 GGCGGGCTGG 10 147 0 TGCAAGTGGG 10 676 0 GGTTGAGGGG 10 807 0 GGGTGGAGAG 10 818 0 TTTAGGCGGG 10 838 0 TTAAGAGGGG 10 897 1 TTAAGAGGGG 10 1173 0 ********** MAP Score: 7.48357 Motif 12 AAGAGGGGGAAGAAAT 0 1120 1 AAGAGAAAGAATGGGA 0 1153 1 ATGAGGGGAAAAGTCA 0 1653 1 ATGGGGAGAAAAGTCA 1 142 0 GAGGGAGTGGATCTTA 2 402 0 AAGAGGAAAAAGGTGA 2 726 1 ACGGGGGGAAACTCAA 4 341 1 GAGGGGGGCCACTTTA 4 444 1 GAGGGGAGCCAAAACC 4 467 1 GAGAGATAGAAAAGTA 4 594 1 GAGAGCCTGTACAGAA 4 717 1 GAGTGCATGCATGAAA 5 137 1 GTGGGATAGCAGAAAA 5 632 1 GAGGGGAATTCAAGTA 5 766 1 AAGGGGGGGAAGTTTA 5 990 1 GAGAGAAGAGATAAAA 5 1035 1 AAGGGCAAGAAGCCAA 6 562 0 GAGGGGTAAACCATTA 6 1008 1 GAGGGGTTGTAGAAAA 7 203 0 GCAGGGAAGAAAGTTA 7 510 1 GAGAGAGTCAAAAAAA 10 230 1 GAGGGGCAAAAGTTAA 10 797 0 GAGGGGTTGGATAACA 10 901 1 GAGAGGTACCATATTA 10 966 1 GAGGGGTTAAAACGCT 10 1163 0 GCGGGGCGCGCATGAA 11 137 0 ****** ** * * MAP Score: 5.04273 Motif 13 AAGAATGGGAAG 0 1159 1 AAAATTGAAGGG 0 1362 1 ATTTATGAGGGG 0 1649 1 AATGTTGAGAAG 1 115 1 AATTTTGATGGG 1 153 0 AGTTTTGGTGGG 2 14 0 TATATTGAGGGG 2 697 1 AATTGTGAGGAG 2 937 1 TATATTGATAGG 3 112 0 CGAATTGAGGAG 4 154 0 AATATGGAGGGG 4 461 1 AAAGTAGAGGAG 4 545 1 AAATTTGGGAGG 5 290 1 AATCTGGAGAAG 5 578 1 AATATTGTGAGG 5 592 1 AATATTGAGAAG 5 650 1 AACTAAGAGGGG 5 760 1 ATCGGTGAGACG 5 797 1 AAGGGGGGGAAG 5 990 1 AGGTGAGAGAAG 5 1031 1 AAAAGAGAGAAG 6 708 1 AATTTTGATGAG 6 943 1 AGAAGGGAGGGG 6 1002 1 AGCGATGAGGGG 7 213 0 TATTTTGAGGAG 10 448 0 AAAATTGATAAG 10 606 1 AAAGAAGAGAGG 10 960 1 AAAGTTGGAAAG 10 1240 1 ** ******** MAP Score: 2.78422 Motif 14 GAGGGAAGTT 0 1240 1 TGGGGGAATT 2 131 1 GAGGGGAGTT 2 703 1 GGGGGAAACT 4 344 1 GAGGGGGGCC 4 444 1 GAGGGGAGCC 4 467 1 ACAGGGAGTT 4 651 1 ATGGGGGGTT 5 608 1 GAGGGGAATT 5 766 1 GGGGGGAAGT 5 993 1 GTGGGGAACT 11 110 1 ********** MAP Score: 2.59839 Motif 15 AGAATGAGAATT 0 511 0 ATAATGAGAATT 2 50 0 AAATTTAGAATT 3 612 1 AAACTGAGAATA 4 571 0 ATACTGAGAATA 6 225 1 AAAGTGAGAATA 7 50 0 AAAGTTAGAATA 7 519 1 AAAATGAGAATA 10 368 0 ATAATTAGAATA 10 1109 0 * * ******** MAP Score: 1.70327 Motif 16 AAAGAAAGAAA 0 268 1 ACAAGAAAAAA 0 324 0 AAAAGAAGAAA 0 462 0 AAAAGGGAGAA 0 1099 1 AAGAGGGGGAA 0 1120 1 GAAAGAAAGAA 0 1136 1 AAGAATGGGAA 0 1159 1 GGAAGAAGGAA 0 1181 1 ACATGAGGGAA 0 1236 1 AAAGAAAAAAA 1 105 1 GCAGGCGGGAA 2 82 1 AAAAGGAAAAA 2 192 0 GAAGATGGAAA 2 365 1 AAAGGCGGGAA 2 507 1 AAAAGTAGAAA 3 259 1 GCAAGTGGAAA 4 181 1 ACAAGGGGGAA 4 316 0 ACGGGGGGAAA 4 341 1 GAATAAGGGAA 4 565 0 AAAAAAAGAAA 5 69 0 AAAAAAAGGAA 5 243 1 AAGGGGGGGAA 5 990 1 GCAGGTGAGAG 5 1029 1 AAGGGCAAGAA 6 567 0 AAAAGAGAGAA 6 708 1 GAAAGTAAAAA 6 1135 1 AAAAGTGAGAA 7 52 0 AAAAAAAGAAA 7 376 0 ATAAGAAAAAA 7 467 1 GCAGGGAAGAA 7 510 1 GCAAGGGGGAA 8 39 1 AAAAGGAGGAA 9 76 1 AAAAGAAAAAA 10 299 0 AAAAATGAGAA 10 370 0 GCAAGTGGGAA 10 674 0 ***** ***** MAP Score: 1.44803 Motif 17 GAAAATAAATTACGTG 0 861 0 AATGGAAAAAAGGGAG 0 1092 1 GAGGAATAAGAGGGGG 0 1113 1 GAGAAAGAATGGGAAG 0 1155 1 AGGAAACAATGTAGTG 0 1187 1 AGGGGAAAAGTCAATG 0 1656 1 GGGAATTAATTTGAAG 2 134 1 AAGAGTAAAAAAGGAG 2 247 0 GGGAAAAAAGGTGGAA 2 288 0 AAAGAGGAAAAAGGTG 2 725 1 GAAGACTATTTCGGAG 2 749 1 AAAGAACAAGTCAGTG 3 640 0 AGGAACAAACAAGGGG 4 319 0 AAGACATATTTTGGAG 4 529 1 AAGAAAAAAATTAATG 5 59 0 GAGGGTTAATGGGGGG 5 600 1 GGGGGTAATTGTTGAG 5 825 0 AAGAAAAAAATAAGTG 7 469 1 AGGGAATAATAGCAGG 7 499 1 GGGGAAAAATTTAGCG 8 44 1 GAGAGTCAAAAAAAGG 10 232 1 GAGAAAAAAAAATGAG 10 372 0 GGGGAAAATTTAAGAG 10 1176 0 GGGAACTAAGGAGACG 11 113 1 ***** ** ** * MAP Score: 0.238573