AlignACE 4.0 05/13/04 ./AlignACE -i cparvum_hsp.fa -numcols 10 -gcback 0.27 Parameter values: expect = 10 gcback = 0.27 minpass = 200 seed = 1181608853 numcols = 10 undersample = 1 oversample = 1 Input sequences: #0 cgd2_20 heat shock 70 (HSP70) protein #1 cgd2_1800 heat shock 40 kDa protein, putative #2 cgd2_3230 heat shock protein DnaJ Pfj2, putative #3 cgd3_3440 heat shock protein HSP70, mitochondrial #4 cgd2_3330 APG-1 like HSP70 domain containing protein, signal peptide plus likely ER retention motif #5 cgd3_3770 Hsp90 #6 cgd4_3270 heat shock 105kD; heat shock 105kD alpha; heat shock 105kD beta; heat shock 105kDa protein 1 #7 cgd6_1090 DnaJ(hsp40)'DnaJ(hsp40)' #8 cgd6_2650 heat shock protein, putative #9 cgd6_4970 Hsp60; GroEL-like chaperone (ATpase), predicted mitochondrial #10 cgd7_360 heat shock protein, Hsp70 #11 cgd7_3670 heat shock protein 90 (Hsp90), signal peptide plus ER retention motif Motif 1 AAGGGGGGGA 5 990 1 AAGAGGGGGA 0 1120 1 AAGAGGGGAA 5 764 1 ATGAGGGGAA 0 1653 1 AAGGCGGGAA 2 508 1 AAGTGGGGGA 6 850 1 AAGGGGGAAA 8 41 1 ATTGGGGGAA 2 129 1 ATGGGGGGTT 5 608 1 ATGGGGGAGG 2 414 0 ATGTGGGGAA 11 108 1 TACGGGGGGA 4 340 1 AACAGGGGGA 6 691 1 AAGGGAGGGG 6 1004 1 TTGGCGGGAA 9 13 1 TTGAGGGGCA 10 805 0 TTGAGGGGAG 2 701 1 AATGGGGGTA 5 834 0 AAGGGGGAAT 4 315 0 TAGGCGGGCA 10 836 0 ATGGAGGGGA 4 464 1 AAGAGGGGTT 10 899 1 AAGAGGGGTT 10 1171 0 TTTGGGGGAA 10 1186 0 CAGGCGGGAA 2 83 1 ATGAGGGGTT 7 211 0 AATAGGGGCA 5 1022 1 ATTAGGGGGT 0 1444 1 TTGGGAGGAA 5 294 1 ATGAGGGAAG 0 1238 1 AAGTGGGAAA 10 673 0 TTGGCGGGCT 10 149 0 AAGGGAGAAA 0 1101 1 AAGAGAGGTA 10 964 1 AACTGGGGAG 2 309 0 ATGGGGAGAA 1 148 0 ********** MAP Score: 33.7003 Motif 2 GCAAGGGGGA 8 39 1 GCGAGGGGGG 4 442 1 ACAAGGGGGA 4 317 0 AAGGGGGGGA 5 990 1 GCAGGCGGGA 2 82 1 AAGAGGGGGA 0 1120 1 GCAAGTGGGA 10 675 0 ACGGGGGGAA 4 341 1 ACAGGGGGAA 6 692 1 GGGGGAGGGA 2 412 0 AAGAGGGGAA 5 764 1 GCAAGTGGAA 4 181 1 GCAGGTGAGA 5 1029 1 AAAGGCGGGA 2 507 1 GCGAGGGAAA 2 298 0 AGAAGTGGGG 6 848 1 AAGGGAGGGG 6 1004 1 AAAAGGGAGA 0 1099 1 AGGAGTGGAA 10 54 0 TAGAGTGGGA 5 628 1 AAAGGTGGAA 2 288 0 GCAAGAGAGA 2 322 0 ********** MAP Score: 26.5172 Motif 3 ACAAAAAAAAAA 0 127 0 ATTAAAAAAAAA 0 190 1 ACAAGAAAAAAA 0 323 0 AAAAGAAGAAAA 0 461 0 ATAAAAAAAAAA 0 717 0 ATTAAAAAAAAA 0 752 0 AATAGAAGAAAA 0 872 0 AATAAAAAAAAA 0 1216 1 AAAAGAAAAAAA 1 104 1 AATAGAAAAACA 2 262 0 ATCAGAAGAACA 3 463 1 AAAAGAAAAAAA 5 65 0 AGCAAAAAAAAA 5 482 1 AGTAGAAGAACA 6 813 1 AAAAAAAGAACA 6 1141 1 ATTAAAAAAAAA 7 234 1 AATAAAAAAAAA 7 254 1 ACAAAAAAAAAA 7 380 0 ATAAGAAAAAAA 7 467 1 ATGAAAAAAAAA 9 44 0 ATCAGAAAAAAA 9 95 0 ATTAAAAGAAAA 10 301 0 AGGAGAAAAAAA 10 378 0 * ********* MAP Score: 18.8244 Motif 4 ATCTAAGGAAAAGGTTC 10 403 1 AACAAAGAAAAAGGAAA 2 194 0 AAAGAAATAAAAGGCAA 0 233 0 AAAGAATCAAAAGGTAA 6 493 0 ATTAAAAAAAAAGGCCA 0 747 0 ACGGAATTAAAAGGTAA 2 789 1 ACATAAAAAAAAGGAAA 5 238 1 AATTAAAGAAAAGGAGG 9 68 1 AAGCAAGCAAAAGGCTG 4 362 0 ATATAATAAAATGGTAA 0 806 0 ATAAAAGTAAATGGAAA 0 1083 1 ATAAAAAAAAATGGACG 0 1217 1 AGTCAAAAAAAGGGATC 10 235 1 TACCAAGCAAAAGGTAC 6 1027 1 TAGCAATAAAAAGGCTC 2 157 0 TGCCAAATAAATGGTGA 5 954 1 TATCAAAAAAAGGGTAA 5 1057 1 ACTAAAAAATAAGGCGC 8 57 0 * ** ****** * MAP Score: 13.646 Motif 5 GAGGGGGAAGAAA 0 1122 1 GGGAAGAGTAGTA 0 1165 1 GGGAAGTTTGAAA 0 1242 1 GAAGGGTTTAGGC 0 1368 1 GAGGGGAAAAGTC 0 1655 1 GGGGAGAAAAGTC 1 143 0 GGCAGGCGGGAAC 2 81 1 GGCGAGGGAAAAA 2 296 0 GGGAAGATGGAAA 2 363 1 GGGAGGGAGTGGA 2 407 0 GGGAGGTTTTACC 2 668 0 GAGGGGAGTTTCA 2 703 1 GGAAAGAGGTCAA 3 202 1 GAAAGGTGGTCAA 4 3 0 GGGGGGAAACTCA 4 343 1 GCGAGGGGGGCCA 4 442 1 GGAGGGGAGCCAA 4 466 1 GAGGAGTTTGGAA 4 551 1 GGAGAGATAGAAA 4 593 1 GGGGGGTTACGAC 5 610 1 GGGGGGGAAGTTT 5 992 1 GGCAGGTGAGAGA 5 1028 1 GAAGTGGGGGATA 6 849 1 GAAGGGAGGGGTA 6 1003 1 GAGGGGTTGTAGA 7 206 0 AGCAGGGAAGAAA 7 509 1 GGCAAGGGGGAAA 8 38 1 GAAAAGGAGGAAC 9 75 1 GGCGGGCTGGCAC 10 144 0 GAGGAGTTGATGA 10 441 0 GGAGAGCGGTTGA 10 811 0 GAGGGGTTGGATA 10 901 1 GAAGAGAGGTACC 10 963 1 GAGGGGTTAAAAC 10 1166 0 ****** *** * MAP Score: 11.0854 Motif 6 GGGGAAGAAATGAA 0 1125 1 GAGGGAAGTTTGAA 0 1240 1 GGGGAAAAGTCAAT 0 1657 1 GGGAGAAAAGTCAA 1 141 0 GGCGGGAACATATA 2 85 1 GGGGAGGGAGTGGA 2 407 0 GAGGGGAGTTTCAG 2 703 1 GGGGGGAAACTCAA 4 343 1 GGGGGCCACTTTAA 4 447 1 GGAGGGGAGCCAAA 4 466 1 GGGAGGAATACAAA 5 296 1 GAGGGGAATTCAAG 5 766 1 GGGGGGGAAGTTTA 5 992 1 GGGCAGGATTTAAA 6 303 0 GGGCAAGAAGCCAA 6 562 0 GGGCGGTAAACCAT 6 651 0 GGGGAACCCTCTAA 6 696 1 GGGGGATATTGTAA 6 854 1 GGGAACAATTTAAA 6 1094 1 GGGAAGAAAGTTAG 7 513 1 GGGGGAAAAATTTA 8 43 1 GGGCAAAAGTTAAA 10 796 0 GGGGAAAATTTAAG 10 1178 0 GGGGCGCGCATGAA 11 137 0 ****** * * ** MAP Score: 9.79789 Motif 7 AAATGGAAAAAAGGG 0 1091 1 TGAGGAATAAGAGGG 0 1112 1 TAAAAATTAGGGGGT 0 1439 1 TACGTGGCAGGCGGG 2 76 1 TGCAACTGGGGAGGG 2 307 0 TGGGGGAGGGAGTGG 2 408 0 TATATATTGAGGGGA 2 695 1 GGAAAGTTAGTAGGG 3 99 0 GAACAAACAAGGGGG 4 318 0 TAAAATACGGGGGGA 4 335 1 TTTCCGCGAGGGGGG 4 437 1 TAAAATATGGAGGGG 4 458 1 TGAGGGTTAATGGGG 5 599 1 TATAACTAAGAGGGG 5 757 1 TATCGGTGAGACGGG 5 796 1 TATTTTAAATGGGGG 5 836 0 GATAAGTCAAGGGGG 5 982 1 TAAAAGAGAGAAGGG 6 707 1 TGGATAGAAGTGGGG 6 843 1 TGCAGAAGGGAGGGG 6 999 1 TGAGCGATGAGGGGT 7 212 0 GGTATGGCAAGGGGG 8 33 1 TAAAGAAAAGGAGGA 9 71 1 GAGCGGTTGAGGGGC 10 806 0 TAATGTTTAGGCGGG 10 838 0 TAAATTTAAGAGGGG 10 892 1 GAAAATTTAAGAGGG 10 1174 0 ** * ******* MAP Score: 9.42617 Motif 8 GCCAAAAGTGCGAG 0 346 0 GAAAAAAGAAGAAA 0 462 0 GAAAAAAGGGAGAA 0 1096 1 GAAAGAAAGAAAAA 0 1136 1 GCAATAAAAAAAAA 0 1214 1 GAAAAAAGGTGGAA 2 288 0 GAAATAAGTACGAA 2 640 1 GCCATGAATTTAAA 2 987 0 GAAATAAAAAAAAG 3 526 1 GCAATAAGTTAAAG 3 565 1 GAAAAGAAGCTGAG 4 86 0 GCAATAAAACAGAA 4 752 1 GACATAAAAAAAAG 5 237 1 GAAATAAAGGTAAA 5 251 1 GCAATAAATTCGAA 6 325 1 GCAAAGAATCAAAA 6 498 0 GCCAAAAGTGAGAA 7 52 0 GCAATAAAAAAGAA 8 18 0 GAAAAAAAAATGAG 10 372 0 GAAATAAAGGAAAG 10 755 0 **** *** *** MAP Score: 6.99846 Motif 9 GGCGCCAAAAG 0 352 0 GGCGCCAAATC 0 1378 1 GGCGGTAATTC 0 1456 1 GGCGGGAACAT 2 85 1 GGCTCCCAAAT 2 151 0 GGCGGGAACAT 2 510 1 GGCTTTAGACG 3 17 0 GGCGCCATAGG 3 475 0 GGAGCCAAAAC 4 471 1 GGCGGTAAACC 6 653 0 GGCGCCAACAG 6 671 1 GGCGCTAAATT 8 51 0 GGCGGGAAAAG 9 15 1 AGCTCTAGAAG 10 269 0 ********* * MAP Score: 5.80052 Motif 10 AAAATGAGGAATAAGAGGG 0 1108 1 AAGGAAACAATGTAGTGGG 0 1186 1 GTCTAAAAATTTTGATGGG 1 153 0 GCGGTTAAGTTTTGGTGGG 2 14 0 GAGATGCAACTGGGGAGGG 2 307 0 GTGTAGTGATGGGGGAGGG 2 413 0 ATGGTTTGGTTTTGGCGGG 10 151 0 GAGTTGATGAGTTGCAGGG 10 432 0 GTGGAGAGCGGTTGAGGGG 10 807 0 GGGGGAAAATTTAAGAGGG 10 1174 0 ATCGAACAGTATCGCGGGG 11 147 0 * ** * * ** *** MAP Score: 3.3879 Motif 11 AAAGTTTAATTA 0 888 0 AAAGTTTAGTTT 1 287 0 AAAGTTTAATTT 2 447 1 AAAGTTAAGTTT 4 517 0 AAAGGTAAATTT 5 256 1 ACAGTTAAGTTT 5 434 1 AAAGGGTAATTT 5 1065 1 AAAGTTGAATTT 6 339 0 AAAGTTAATTTT 6 937 1 AAAGTGTAATTT 10 355 0 AAAGTTGAGTTT 10 567 0 AAAGTGAAATTT 10 622 1 ****** * *** MAP Score: 3.03759 Motif 12 CTACATGAGGGAAGTTT 0 1234 1 GGTTTGGCGGTAATTCT 0 1451 1 TTTATGAGGGGAAAAGT 0 1650 1 TTGATGGGGAGAAAAGT 1 144 0 GTTTTGGTGGGCCGAAT 2 8 0 TGGCAGGCGGGAACATA 2 80 1 GGGAGGGCGAGGGAAAA 2 297 0 ATGGGGGAGGGAGTGGA 2 407 0 GGAAAGGTGGTCAATCT 4 0 0 CGCGAGGGGGGCCACTT 4 441 1 AATATGGAGGGGAGCCA 4 461 1 GTAACGGTGATAAGTTA 5 208 1 GTTAGAGTGGGATAGCA 5 626 1 GTATCGGTGAGACGGGT 5 795 1 GTCAAGGGGGGGAAGTT 5 987 1 GGGCAGGTGAGAGAAGA 5 1027 1 GTTTGGGCGGTAAACCA 6 652 0 CTAATTGTGGTCTTAAT 7 587 0 GGCAAGGGGGAAAAATT 8 38 1 ATTTTGGCGGGAAAAGT 9 10 1 GTTTTGGCGGGCTGGCA 10 145 0 GTTGAGGGGCAAAAGTT 10 799 0 GTTTAGGCGGGCATTAT 10 832 0 CGCGGGGCGCGCATGAA 11 137 0 ** ******* * MAP Score: 2.68361 Motif 13 GGAAAAAAGGGA 0 1095 1 AATAAGAGGGGG 0 1117 1 GAAAGAATGGGA 0 1157 1 GTGAGATGGGGT 0 1705 0 TTTTGATGGGGA 1 151 0 GGCAGGCGGGAA 2 81 1 AAAAAGGTGGAA 2 288 0 GGGAAGATGGAA 2 363 1 GGAGGGAGTGGA 2 407 0 AAAAGGCGGGAA 2 506 1 GGGTAAAGTGAA 2 778 1 GTGAGGAGTGGT 2 941 1 AACAAGGGGGAA 4 316 0 ATATGGAGGGGA 4 462 1 GGTAAACAGGGA 4 646 1 GAAAGAATGGGA 5 274 1 GGTTAATGGGGG 5 603 1 GTTAGAGTGGGA 5 626 1 GTGAGACGGGTA 5 801 1 GTCAAGGGGGGG 5 987 1 TGCAGAAGGGAG 6 999 1 GGTAAAATGGGA 6 1086 1 GGCAAGGGGGAA 8 38 1 GGCGGGCTGGCA 10 145 0 GACAGATTGGGA 10 205 1 GGAAAGATTGAA 10 749 0 GGTTGAGGGGCA 10 805 0 TTTAGGCGGGCA 10 836 0 ** *** ***** MAP Score: 0.680181