AlignACE 4.0 05/13/04 ./AlignACE -i cparvum_hsp.fa -numcols 10 -gcback 0.27 Parameter values: expect = 10 gcback = 0.27 minpass = 200 seed = 1181608828 numcols = 10 undersample = 1 oversample = 1 Input sequences: #0 cgd2_20 heat shock 70 (HSP70) protein #1 cgd2_1800 heat shock 40 kDa protein, putative #2 cgd2_3230 heat shock protein DnaJ Pfj2, putative #3 cgd3_3440 heat shock protein HSP70, mitochondrial #4 cgd2_3330 APG-1 like HSP70 domain containing protein, signal peptide plus likely ER retention motif #5 cgd3_3770 Hsp90 #6 cgd4_3270 heat shock 105kD; heat shock 105kD alpha; heat shock 105kD beta; heat shock 105kDa protein 1 #7 cgd6_1090 DnaJ(hsp40)'DnaJ(hsp40)' #8 cgd6_2650 heat shock protein, putative #9 cgd6_4970 Hsp60; GroEL-like chaperone (ATpase), predicted mitochondrial #10 cgd7_360 heat shock protein, Hsp70 #11 cgd7_3670 heat shock protein 90 (Hsp90), signal peptide plus ER retention motif Motif 1 GATAAGTCAAGGGGGG 5 982 1 GTTGAGCGATGAGGGG 7 213 0 GGATAGAAGTGGGGGA 6 844 1 GGTATGGCAAGGGGGA 8 33 1 GAGGGTTAATGGGGGG 5 600 1 GAATTTCCGCGAGGGG 4 434 1 GTGTAGTGATGGGGGA 2 416 0 ATGCAGAAGGGAGGGG 6 998 1 GTATCGGTGAGACGGG 5 795 1 GAAAATTTAAGAGGGG 10 1173 0 GTTTGGTTTTGGCGGG 10 151 0 GAACAGTATCGCGGGG 11 147 0 GAGACGATGTGGGGAA 11 102 1 GAACAAACAAGGGGGA 4 317 0 GAGCGGTTGAGGGGCA 10 805 0 ATATAACTAAGAGGGG 5 756 1 AGCTATTTATGAGGGG 0 1645 1 GGAGAAAGTAGAGGAG 4 541 1 CTTAAAATACGGGGGG 4 333 1 ATAAATTTAAGAGGGG 10 891 1 GTTAAGTTTTGGTGGG 2 14 0 TGAGGAATAAGAGGGG 0 1112 1 GATAAAATAAGAGGCG 6 543 0 GACGCTACATGAGGGA 0 1230 1 GTATCTATTTGAGGAG 10 385 0 AAAATATGGAGGGGAG 4 459 1 ATCGCGAATTGAGGAG 4 154 0 GGGCAGGTGAGAGAAG 5 1027 1 TTTATATATTGAGGGG 2 693 1 TTAATGTTTAGGCGGG 10 838 0 GATGCAACTGGGGAGG 2 308 0 AGTAGATATTGGGGGA 2 122 1 AAATTTTGATGGGGAG 1 150 0 ATAAGAAAACGAGGAG 0 1043 1 ** * * ****** MAP Score: 35.2017 Motif 2 GCGAGGGGGGCCA 4 442 1 GCAAGGGGGAAAA 8 39 1 GCAGGCGGGAACA 2 82 1 AAGAGGGGGAAGA 0 1120 1 GAGGGCGAGGGAA 2 299 0 AAAGGCGGGAACA 2 507 1 GGAGGGGAGCCAA 4 466 1 AACAGGGGGAACC 6 691 1 GGGAGGGAGTGGA 2 407 0 GAAGTGGGGGATA 6 849 1 AATGGGGGGTTAC 5 607 1 AAGGGGGGGAAGT 5 990 1 GATGGGGAGAAAA 1 146 0 GCGGGCTGGCACA 10 143 0 GAAGGGAGGGGTA 6 1003 1 GAAAGGTGGTCAA 4 3 0 TACGGGGGGAAAC 4 340 1 GAGATGGGGTAAC 0 1702 0 AAAAGGGAGAAAA 0 1099 1 AACAGGGAGTTTA 4 650 1 ACAAGGGGGAATT 4 314 0 AAAAGGTGGAATA 2 286 0 ACAAGCGAGCGAA 7 154 1 TGAGGGGAGTTTC 2 702 1 GCAGGTGAGAGAA 5 1029 1 AAGAGGGGTTAAA 10 1168 0 AAGAGGGGTTGGA 10 899 1 ********* * MAP Score: 21.0176 Motif 3 AATAGATAAGTCAAGGGGGGGA 5 978 1 AAGTGTGTAGTGATGGGGGAGG 2 414 0 TGGCGCCTAGTTTGGGCGGTAA 6 656 0 AATTGAAGGGTTTAGGCGCCAA 0 1364 1 TGCCGGTATGGCAAGGGGGAAA 8 29 1 ATAATTAATGTTTAGGCGGGCA 10 836 0 AATGGTTTGGTTTTGGCGGGCT 10 149 0 ATTTGGATAGAAGTGGGGGATA 6 840 1 AGAAGAACACCTATGGCGCCAA 3 466 1 AAATTAGGGGGTTTGGCGGTAA 0 1442 1 TGGAGAGCGGTTGAGGGGCAAA 10 803 0 AATCGAACAGTATCGCGGGGCG 11 145 0 TGCGGTTAAGTTTTGGTGGGCC 2 12 0 AGGAATTTCCGCGAGGGGGGCC 4 432 1 ACCGGGTAGGTTTTGGCTCCCC 4 469 0 ATTTACGCCCAAAAGGCGGGAA 2 496 1 AAAAGCAAGCAAAAGGCTGAAA 4 359 0 ATTCTCGCACTTTTGGCGCCAA 0 343 1 AAGAGAGATGCAACTGGGGAGG 2 308 0 TATATATATGGAAAGGTGGTCA 4 4 0 ATGTTACGTGGCAGGCGGGAAC 2 72 1 * * * ***** ** MAP Score: 20.591 Motif 4 ACAAAAAAAAAA 0 127 0 ATTAAAAAAAAA 0 190 1 ACAAGAAAAAAA 0 323 0 AAAAGAAGAAAA 0 461 0 ATAAAAAAAAAA 0 717 0 ATTAAAAAAAAA 0 752 0 AATAGAAGAAAA 0 872 0 AATAAAAAAAAA 0 1216 1 AAAAGAAAAAAA 1 104 1 AATAGAAAAACA 2 262 0 ATCAGAAGAACA 3 463 1 AAAAGAAAAAAA 5 65 0 AGCAAAAAAAAA 5 482 1 AGTAGAAGAACA 6 813 1 AAAAAAAGAACA 6 1141 1 AAAAAAAAAAAA 7 237 1 AATAAAAAAAAA 7 254 1 ACAAAAAAAAAA 7 380 0 ATAAGAAAAAAA 7 467 1 ATGAAAAAAAAA 9 44 0 ATCAGAAAAAAA 9 95 0 ATTAAAAGAAAA 10 301 0 AGGAGAAAAAAA 10 378 0 * ********* MAP Score: 18.8244 Motif 5 AAGTAAATGGAAAAAAGGGAGAA 0 1087 1 AATGAGGAATAAGAGGGGGAAGA 0 1110 1 GTAGTAGTAGGAAGAAGGAAACA 0 1172 1 ATTTATCTAGTGAGATGGGGTAA 0 1703 0 GTCTAAAAATTTTGATGGGGAGA 1 149 0 GCGGTTAAGTTTTGGTGGGCCGA 2 10 0 ATGTTACGTGGCAGGCGGGAACA 2 72 1 GATGCAACTGGGGAGGGCGAGGG 2 301 0 GTAGTGATGGGGGAGGGAGTGGA 2 407 0 GTTTTAGGCTTTAGACGAGCAAA 3 11 0 GGCTTTATAGTGTACCGGGTAGG 4 481 0 AGGGTATCGGTGAGACGGGTACG 5 792 1 ATAGATAAGTCAAGGGGGGGAAG 5 979 1 GGGGCAGGTGAGAGAAGAGATAA 5 1026 1 GGGGAACCCTCTAAAAGAGAGAA 6 696 1 ACTTTTTCATGCAGAAGGGAGGG 6 990 1 GCCGGTATGGCAAGGGGGAAAAA 8 30 1 ATGGTTTGGTTTTGGCGGGCTGG 10 147 0 GTTGCAGGGTTGAGCTGAGATGA 10 418 0 GTTTTCCTATTTTGAGGAGTTGA 10 444 0 GTGGAGAGCGGTTGAGGGGCAAA 10 803 0 GGGGAAAATTTAAGAGGGGTTAA 10 1169 0 ATCGAACAGTATCGCGGGGCGCG 11 143 0 * * * ** *** ** MAP Score: 18.4057 Motif 6 GATGAGGGGTTGTAGAAAA 7 203 0 GGGGAGGGCGAGGGAAAAA 2 296 0 GTCAAGGGGGGGAAGTTTA 5 987 1 TAAGAGGGGTTGGATAACA 10 898 1 TTTGATGGGGAGAAAAGTC 1 143 0 GTTGAGGGGCAAAAGTTAA 10 797 0 TAAGAGGGGGAAGAAATGA 0 1119 1 GGGGAGGGAGTGGATCTTA 2 402 0 TTTGGCGGGCTGGCACACA 10 141 0 TATGGAGGGGAGCCAAAAC 4 463 1 GTTAATGGGGGGTTACGAC 5 604 1 TTTGGTGGGCCGAATAAAA 2 4 0 GGCAAGGGGGAAAAATTTA 8 38 1 GCGAGGGGGGCCACTTTAA 4 442 1 GATGAAGCGCAGAATTTAA 2 809 0 TAAGAGGCGTGGTTTTTGC 6 533 0 GGAGACGCCGTGTTATTCA 11 122 1 TGAGATGGGGTAACTTGTA 0 1697 0 GCAGAAGGGAGGGGTAAAC 6 1000 1 TGAGACGGGTACGCAAGTA 5 802 1 GTTGGCGCCTAGTTTGGGC 6 661 0 * ******* * * MAP Score: 14.6921 Motif 7 GGTGAAAGGTATA 0 525 1 GAAAAAAGGGAGA 0 1096 1 GAAAGAATGGGAA 0 1157 1 GTGAGATGGGGTA 0 1704 0 GGCAGGCGGGAAC 2 81 1 GAAAAAAGGTGGA 2 289 0 CTGGGGAGGGCGA 2 304 0 GGGAAGATGGAAA 2 363 1 GAGGGAGTGGATC 2 405 0 AAAAGGCGGGAAC 2 506 1 GAAAAGTGGTATC 2 898 1 GGAAAGAGGTCAA 3 202 1 GAAAGGTGGTCAA 4 3 0 GGAAAAAGGTAGA 4 187 1 CGCGAGGGGGGCC 4 441 1 TTAGAGTGGGATA 5 627 1 GGTGAGACGGGTA 5 800 1 GTCAAGGGGGGGA 5 987 1 GGCAGGTGAGAGA 5 1028 1 TAGAAGTGGGGGA 6 847 1 GAAGGGAGGGGTA 6 1003 1 GGTAAAATGGGAA 6 1086 1 GAGGGGTTGTAGA 7 206 0 GGCAAGGGGGAAA 8 38 1 GGCGGGCTGGCAC 10 144 0 GACAGATTGGGAA 10 205 1 TGCAAGTGGGAAA 10 673 0 GGTTGAGGGGCAA 10 804 0 GAGGGGTTGGATA 10 901 1 GAAGAGAGGTACC 10 963 1 GACGATGTGGGGA 11 104 1 ** *** **** * MAP Score: 13.8759 Motif 8 TTTGGCGCCAA 0 354 1 CATGGCGGTTA 0 997 1 TTAGGCGCCAA 0 1375 1 TTTGGCGGTAA 0 1453 1 TATTGGGGGAA 2 128 1 AAAGGCGGGAA 2 507 1 TATGGCGCCAA 3 477 1 TACGGGGGGAA 4 340 1 TTTGGGAGGAA 5 293 1 TAAGAGGGGAA 5 763 1 TTAGTCGCGAA 6 463 1 TTGGGCGGTAA 6 656 0 CTAGGCGCCAA 6 668 1 CAAGGGGGAAA 8 40 1 TAAGGCGCTAA 8 54 0 TTTGGCGGGAA 9 12 1 TTTGGCGTAAA 10 347 1 CAAGTGGGAAA 10 673 0 ** ******** MAP Score: 12.984 Motif 9 AGAAAACGAGGAG 0 1046 1 GGAAAAAAGGGAG 0 1095 1 GAATAAGAGGGGG 0 1116 1 AGATATTGGGGGA 2 125 1 AGTAAAAAAGGAG 2 247 0 GAAAAAAGGTGGA 2 289 0 AGTGATGGGGGAG 2 415 0 AAACAAGGGGGAA 4 316 0 GGTAAACAGGGAG 4 646 1 GGTTAATGGGGGG 5 603 1 AGTCAAGGGGGGG 5 986 1 GATAGAAGTGGGG 6 845 1 GAGCGATGAGGGG 7 213 0 AGAAAAGGAGGAA 9 74 1 GACAGATTGGGAA 10 205 1 AGCGGTTGAGGGG 10 807 0 AATTTAAGAGGGG 10 1173 0 AGACGATGTGGGG 11 103 1 ** ** ****** MAP Score: 10.9333 Motif 10 GCATGCAAAA 0 935 0 GCATGCAATC 0 948 1 GCATGAACAA 0 984 1 GCATGCATTC 2 36 0 TCATGCATTC 2 388 1 GCATGCAAAA 3 289 1 GCAAGCAAAA 4 367 0 GCATGCACTC 5 137 0 TCATGCACTA 5 171 0 GCTTGCATAA 5 318 0 GCTTGCATAC 5 360 1 GCATGAAAAA 6 992 0 GCTGGCACAC 10 142 0 GCATGAACAA 10 736 1 GCATGAATAA 11 134 0 ********** MAP Score: 10.6277 Motif 11 AAAGGGAGAAAATGAGG 0 1100 1 GAGGGGGAAGAAATGAA 0 1122 1 AATGGGAAGAGTAGTAG 0 1162 1 AGAAGGAAACAATGTAG 0 1184 1 ATGAGGGAAGTTTGAAA 0 1238 1 ATTAGGGGGTTTGGCGG 0 1444 1 TGGGGGAATTAATTTGA 2 131 1 GATGGGGGAGGGAGTGG 2 408 0 AAGGCGGGAACATGCAG 2 508 1 ATTGAGGGGAGTTTCAG 2 700 1 GTGAGGAGTGGTTTTGA 2 941 1 ACGGGGGGAAACTCAAG 4 341 1 GAGGGGGGCCACTTTAA 4 444 1 TGTGAGGGTTAATGGGG 5 597 1 GAGGGGAATTCAAGTAA 5 766 1 ATGGGGGTAATTGTTGA 5 826 0 AAGGGGGGGAAGTTTAA 5 990 1 AATAGGGGCAGGTGAGA 5 1022 1 AGTGGGGGATATTGTAA 6 851 1 AATGGGAACAATTTAAA 6 1091 1 GCAGGGAAGAAAGTTAG 7 510 1 AAGGGGGAAAAATTTAG 8 41 1 GTGGAGAGCGGTTGAGG 10 809 0 TTTGGGGGAAAATTTAA 10 1179 0 TGTGGGGAACTAAGGAG 11 109 1 * ****** * ** MAP Score: 9.28949 Motif 12 GACGTAGTGCATGATTGA 5 167 1 GATGAGTTGCAGGGTTGA 10 428 0 CCGGTATGGCAAGGGGGA 8 31 1 GAATAATAGCAGGGAAGA 7 502 1 ACATGAGTGCATGCATGA 5 133 1 CATGCATTGCATGCAAAA 0 935 0 GAAGAAATGAAAGAAAGA 0 1128 1 GAATTATTGCATGCAAAA 3 281 1 GATTAAAAGCAAGCAAAA 4 367 0 ACAGAAACGCATGAACAA 10 728 1 AAATAGGGGCAGGTGAGA 5 1021 1 AAAATAAAGCAAGTTTGA 3 312 1 GTTACGTGGCAGGCGGGA 2 74 1 AAATAAAGGAAAGATTGA 10 750 0 CCTGTACAGAAGGAATAA 4 722 1 ATTAGACTGCAAGTGGGA 10 675 0 ATTTGAATGCATGACAAA 2 384 0 CAGTGAATGCATGCACGT 2 32 1 ATTTAAAAGAAAGAAAGA 0 263 1 ** * * *** * ** MAP Score: 8.36099 Motif 13 GGCGCCAAAAGTGCGAGA 0 345 0 TGAGGAATAAGAGGGGGA 0 1112 1 GAGAAAGAATGGGAAGAG 0 1155 1 GTAGTAGGAAGAAGGAAA 0 1175 1 GACGCTACATGAGGGAAG 0 1230 1 GCTATTTATGAGGGGAAA 0 1646 1 GTTACGTGGCAGGCGGGA 2 74 1 GAGGGAAAAAAGGTGGAA 2 288 0 GATGCAACTGGGGAGGGC 2 306 0 GTAGTGATGGGGGAGGGA 2 412 0 TACGCCCAAAAGGCGGGA 2 499 1 TATATTTATATAGGGAGG 2 675 0 TTTATATATTGAGGGGAG 2 693 1 GGAACAAACAAGGGGGAA 4 316 0 TTAAAATACGGGGGGAAA 4 334 1 GGAATTTCCGCGAGGGGG 4 433 1 TTTAAAATATGGAGGGGA 4 456 1 TTGGAGAAAGTAGAGGAG 4 539 1 TAAGAAATTTGGGAGGAA 5 286 1 GTGAGGGTTAATGGGGGG 5 598 1 GACGTTGTTAGAGTGGGA 5 620 1 TAGATAAGTCAAGGGGGG 5 980 1 GCAGTTGCATGGGCAGGA 6 309 0 TATAATAAACAGGGGGAA 6 684 1 TTTGGATAGAAGTGGGGG 6 841 1 GGTATGGCAAGGGGGAAA 8 33 1 TAAGATATAGGAGTGGAA 10 54 0 GAGAGCGGTTGAGGGGCA 10 805 0 GGGAAAATTTAAGAGGGG 10 1173 0 TTGAGACGATGTGGGGAA 11 100 1 * * ******** MAP Score: 7.24762 Motif 14 AGGGAGAAAATGAG 0 1102 1 AGGGGGAAGAAATG 0 1123 1 AGGGGAAAAGTCAA 0 1656 1 GGGAGAAAAGTCAA 1 141 0 GGGCAAACAATCAG 1 321 0 GGGCCGAATAAAAA 2 3 0 GGCGGGAACATATA 2 85 1 GGGCGAGGGAAAAA 2 296 0 GGGGAGGGAGTGGA 2 407 0 GGGCGTAAATTTAA 2 492 0 GGCGGGAACATGCA 2 510 1 GAGGGGAGTTTCAG 2 703 1 AGGGGGAATTTGTA 4 310 0 GGGGGGAAACTCAA 4 343 1 GGGGGGCCACTTTA 4 446 1 GGAGGGGAGCCAAA 4 466 1 GGGAGGAATACAAA 5 296 1 GAGGGGAATTCAAG 5 766 1 GGGGGGGAAGTTTA 5 992 1 GGGGCAGGTGAGAG 5 1026 1 GGGCAGGATTTAAA 6 303 0 GGGCGGTAAACCAT 6 651 0 GGGGGAACCCTCTA 6 695 1 GGGGGATATTGTAA 6 854 1 GGGGGAAAAATTTA 8 43 1 AGGAGAAAAAAAAA 10 376 0 GGGCAAAAGTTAAA 10 796 0 GGAGAGCGGTTGAG 10 810 0 GGGGAAAATTTAAG 10 1178 0 GGGGCGCGCATGAA 11 137 0 ******** ** MAP Score: 3.47842 Motif 15 AATGAGGAATAAGAGGGGGA 0 1110 1 TTAAATAAAAATTAGGGGGT 0 1434 1 GTTAAGTTTTGGTGGGCCGA 2 10 0 TATGTTACGTGGCAGGCGGG 2 71 1 GTGATGGGGGAGGGAGTGGA 2 407 0 TTAAGGAACAAACAAGGGGG 4 318 0 GAATTTCCGCGAGGGGGGCC 4 434 1 TTAAAATATGGAGGGGAGCC 4 457 1 TGTGAGGGTTAATGGGGGGT 5 597 1 TGAGGGTATCGGTGAGACGG 5 790 1 TAATAGATAAGTCAAGGGGG 5 977 1 TTGGCGCCTAGTTTGGGCGG 6 659 0 GGTTATAATAAACAGGGGGA 6 681 1 TATTTGGATAGAAGTGGGGG 6 839 1 TTTTCATGCAGAAGGGAGGG 6 993 1 GTTGAGCGATGAGGGGTTGT 7 209 0 TTGCCGGTATGGCAAGGGGG 8 28 1 GTTTGGTTTTGGCGGGCTGG 10 147 0 TACACGATCCGTAAAGCTCT 10 274 0 GAGTTGCAGGGTTGAGCTGA 10 423 0 TATATGAATTGGTGAGCTCT 10 542 0 GTGGAGAGCGGTTGAGGGGC 10 806 0 TTCTTGAGACGATGTGGGGA 11 97 1 GAACAGTATCGCGGGGCGCG 11 143 0 * * * ******* MAP Score: 2.59175 Motif 16 ATGGCGGTTAG 0 998 1 ATAAGAGGGGG 0 1118 1 GGGAAGAGTAG 0 1165 1 GTAGGAAGAAG 0 1178 1 AGGGAAGTTTG 0 1241 1 AGGGGGTTTGG 0 1447 1 GGGGAGAAAAG 1 145 0 GTGGCAGGCGG 2 79 1 AGGGCGAGGGA 2 300 0 GGAGGGAGTGG 2 408 0 AGAGGAAAAAG 2 727 1 GTGAGGAGTGG 2 941 1 ATGGAAAGAGG 3 200 1 TGGAAAGGTGG 4 7 0 GGGGGAATTTG 4 312 0 ATGGAGGGGAG 4 464 1 GGAGAAAGTAG 4 541 1 AGGAGAGATAG 4 592 1 AGGGGGGGAAG 5 991 1 GGGGCAGGTGA 5 1026 1 AGGGCAAGAAG 6 566 0 AGAGAGAAGGG 6 711 1 ATAGAAGTGGG 6 846 1 AGAAGGGAGGG 6 1002 1 AGGGAAGAAAG 7 512 1 ATGGCAAGGGG 8 36 1 TTGGCGGGCTG 10 148 0 AGGAAAGATTG 10 751 0 GTGGAGAGCGG 10 815 0 AGAGGGGTTGG 10 900 1 ******** ** MAP Score: 1.54606 Motif 17 AATGGCAAATACTAATCAAAATGT 0 1572 1 AATGTCAAATTATGAATAAGAAAT 0 1612 1 AAAGTCAATGTGTCTCGTAAAGAT 0 1662 1 ATTGTCAAAAATAGATAAAAAGAT 3 441 0 AAAGCCAAAATTAAGGTTAAAACT 4 498 1 AGTGCCAGAAATTAATTTAAATAT 5 713 0 AATGCCAAATAAATGGTGAGATAT 5 952 1 AGAGACAAATCTTATAAAAGATAT 7 129 1 ATTGGCAAACTCCGTGAGAAAATT 10 588 1 AAAGACAAAACAATTAGCAAACTT 10 639 1 * * **** *** * MAP Score: 1.04084 Motif 18 CAAAAAAAAAAA 0 126 0 GCAATAAAAAAA 0 1214 1 GCAATAAAAAGG 2 160 0 CAAAGAAAAAGG 2 197 0 GCAAGCAAAAGG 4 365 0 GCAATAAAACAG 4 752 1 GCAAAAAAAAAT 5 483 1 GCAATAAATATA 5 749 1 GAAAAAAAAATG 6 117 0 GCAACCAAATCG 6 738 0 CCAAGCAAAAGG 6 1029 1 CCAAGCCAAAAG 7 58 0 GAAAAAAAAATA 7 110 0 GAAAAAAATAAG 7 471 1 GCAATAAAAAAG 8 20 0 GAAAAAAAAATG 9 42 0 GAAAAAAAAATG 10 374 0 GCAATCAAAACA 11 65 0 **** ***** * MAP Score: 0.226017