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Data for sequence SLR0741 (spot 22) in slidename rvswtGHL1hr033103 : rvswtGHL1hr033103

1 spot(s) found matching criteria.


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Biological Information
Sequence NameSLR0741
END_COORD131790
LENGTH225
STRAND+
SYSTEMATIC NAMESLR0741
CATEGORYRegulatory functions
GENE_NAMEphoU
PRODUCTa negative regulator of pho regulon
BEG_COORD131116

View expression history of this entity

Spot22
SUID471075
Ch1 Intensity (Mean)10297
Ch1 Background (Median)175
Ch1 Net (Mean)10122
Ch2 Intensity (Mean)13561
Ch2 Net (Mean)13379
Ch2 Background (Median)182
Ch2 Normalized Background (Median)146
Ch2 Normalized Net (Mean)10703
Ch2 Normalized Intensity (Mean)10849
Regression Correlation1
Spot Flag0
% CH1 PIXELS > BG + 1SD91
% CH2 PIXELS > BG + 1SD94
Regression Ratio1.329
Number of Background Pixels914
Number of Spot Pixels156
Box Top69
Box Left511
Box Right526
Box Bottom84
Sum of Ch2 Foreground Pixel Intensities
Sum of Ch1 Foreground Pixel Intensities
Log(base2) of R/G Normalized Ratio (Mean).081
G/R (Mean).757
G/R Normalized (Mean).946
R/G (Mean)1.322
R/G Normalized (Mean)1.057
Channel 2 Background (Mean)1645
Channel 1 Background (Mean)1999
Ch1 Intensity (Median)10591
Ch1 Net (Median)10416
% of saturated Ch1 pixels0
Std Dev of Ch1 Intensity2770
Std Dev of Ch1 Background6320
Ch2 Net (Median)13727
Ch2 Intensity (Median)13909
% of saturated Ch2 pixels0
Std Dev of Ch2 Intensity3797
Std Dev of Ch2 Background5078
Normalized Ch2 Net (Median)10982
Normalized Ch2 Intensity (Median)11127
Diameter of the spot150
R/G Mean (per pixel)1.315
R/G Median (per pixel)1.316
% CH1 PIXELS > BG + 2SD16
% CH2 PIXELS > BG + 2SD84
R/G (Median)1.318
Std Dev of pixel intensity ratios1.105
Sum of mean intensities23501
Sum of median intensities24143
X coordinate (whole array, in microns)6930
Y coordinate (whole array, in microns)3290
Log(base2) of R/G Normalized Ratio (Median).076
R/G Normalized (Median)1.054
Ch2 Signal-to-Noise Ratio
Ch1 Signal-to-Noise Ratio
Ch1 Background Intensity Coefficient of Variation
Ch2 Background Intensity Coefficient of Variation
Ch1 Foreground Intensity Coefficient of Variation
Ch2 Foreground Intensity Coefficient of Variation
Actual Ch1 Background Used in Calculations
Actual Ch2 Background Used in Calculations
Autoflag
Spot Circularity
Channel 1 Mean Intensity / Median Background Intensity58.84
Channel 2 Normalized (Mean Intensity / Median Background Intensity)74.308
Overall Intensity A (sqrt(RG)) (Means)10408.447
Overall Intensity A (sqrt(RG)) (Medians)10695.257


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