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Data for sequence SLL1491 (spot 455) in slidename rvswtGHL1hr033103 : rvswtGHL1hr033103

1 spot(s) found matching criteria.


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Biological Information
Sequence NameSLL1491
SYSTEMATIC NAMESLL1491

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Spot455
SUID502626
Ch1 Intensity (Mean)354
Ch1 Background (Median)195
Ch1 Net (Mean)159
Ch2 Intensity (Mean)446
Ch2 Net (Mean)232
Ch2 Background (Median)214
Ch2 Normalized Background (Median)171
Ch2 Normalized Net (Mean)186
Ch2 Normalized Intensity (Mean)357
Regression Correlation.73
Spot Flag0
% CH1 PIXELS > BG + 1SD95
% CH2 PIXELS > BG + 1SD83
Regression Ratio2.074
Number of Background Pixels475
Number of Spot Pixels156
Box Top476
Box Left372
Box Right387
Box Bottom491
Sum of Ch2 Foreground Pixel Intensities
Sum of Ch1 Foreground Pixel Intensities
Log(base2) of R/G Normalized Ratio (Mean).223
G/R (Mean).685
G/R Normalized (Mean).857
R/G (Mean)1.459
R/G Normalized (Mean)1.167
Channel 2 Background (Mean)225
Channel 1 Background (Mean)200
Ch1 Intensity (Median)355
Ch1 Net (Median)160
% of saturated Ch1 pixels0
Std Dev of Ch1 Intensity68
Std Dev of Ch1 Background35
Ch2 Net (Median)238
Ch2 Intensity (Median)452
% of saturated Ch2 pixels0
Std Dev of Ch2 Intensity132
Std Dev of Ch2 Background96
Normalized Ch2 Net (Median)190
Normalized Ch2 Intensity (Median)362
Diameter of the spot150
R/G Mean (per pixel)1.373
R/G Median (per pixel)1.46
% CH1 PIXELS > BG + 2SD92
% CH2 PIXELS > BG + 2SD61
R/G (Median)1.488
Std Dev of pixel intensity ratios2.181
Sum of mean intensities391
Sum of median intensities398
X coordinate (whole array, in microns)5540
Y coordinate (whole array, in microns)7360
Log(base2) of R/G Normalized Ratio (Median).251
R/G Normalized (Median)1.19
Ch2 Signal-to-Noise Ratio
Ch1 Signal-to-Noise Ratio
Ch1 Background Intensity Coefficient of Variation
Ch2 Background Intensity Coefficient of Variation
Ch1 Foreground Intensity Coefficient of Variation
Ch2 Foreground Intensity Coefficient of Variation
Actual Ch1 Background Used in Calculations
Actual Ch2 Background Used in Calculations
Autoflag
Spot Circularity
Channel 1 Mean Intensity / Median Background Intensity1.815
Channel 2 Normalized (Mean Intensity / Median Background Intensity)2.088
Overall Intensity A (sqrt(RG)) (Means)171.971
Overall Intensity A (sqrt(RG)) (Medians)174.356


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