The instructions that follow are designed to download
the genome sequence of either Streptococcus
pneumoniae TIGR4 or
Streptococcus
mutans. If you sit in the front row, choose S. pneumoniae.
If you sit in the last row, choose S. mutans.
|
|
Click HERE and save the file in the directory of your choice. | File contains 500 nt from each end of 128 large contigs of S. sanguis. Your life will be easier later if you give the file a name with no more than 8 letters (not counting extension). |
Go to TIGR Comprehensive Microbial Resource | A very good site to bookmark. Source of a large number of genomes and information concerning their analysis |
Scroll down to window displaying Genome Pages. Click on arrow, scroll down window, and select either Streptococcus pneumoniae TIGR4 or Streptococcus mutans UA159. | Brings you to page devoted to the organism you're going to download. |
Click on Genbank FTP
|
Brings you to a directory of files within Genbank, one of which you will transfer by File Transfer Protocol. |
Click the file with the .fna extension. Give it a descriptive name and save it in the directory of your choice. Your life will be easier later if you give the file a name with no more than 8 letters (not counting extension). | Maybe fna stands for Fast(nucleotide)A? Anyway, this is the file with the complete nucleotide sequence in FastA format (see below). |
Congratulate self | You're done! |
FastA format
This is a common format for files containing one or more protein or DNA sequences, originally used by the FastA program (a program similar to Blast). The format is:
>[description]
[sequence, in one-letter code if protein. Upper/lower
case doesn't matter.]
Example of a protein sequence in FastA format
>b5937: a mythical protein
MTAQQDPRES...
Example of a set of DNA sequences in FastA format
>zlr4284: a mythical gene
ATGCCCGACGAAGAC...
>rlz4824: another mythical gene
ATGCCCGACGAAGAC...
>...